Invitrogen™ ZCCHC6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA554247

Catalog No. PIPA554247

Description

Immunogen sequence: DDYKGDKVYH PETGRKNEKE KVGRKGKHLL TVDQKRGEHV VCGSTRNNES ESTLDLEGFQ NPTAKECEGL ATLDNKADLD GESTEGTEEL EDSLNHFTHS VQGQ Highest antigen sequence identity to the following orthologs: Mouse - 38%, Rat - 37%.

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (PubMed:30122351). [UniProt].

Specifications

• Antigen: ZCCHC6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ZCCHC6
• Gene Alias: 6030448M23Rik; AA420405; FLJ13409; HS2; KIAA1711; PAP associated domain containing 6; PAPD6; Terminal uridylyltransferase 7; TUT7; TUTase 7; TUTase7; Zcchc6; Zinc finger CCHC domain-containing protein 6; zinc finger CCHC-type containing 6; zinc finger, CCHC domain containing 6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79670
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q5VYS8
• Gene Symbols: ZCCHC6
• Immunogen: Recombinant protein corresponding to Human ZCCHC6. Recombinant protein control fragment (Product #RP-92886).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG