Thermo Scientific™ Zeba™ Spin Desalting Columns, 40K MWCO, 2 mL

Effectively remove salt and other molecules (40K MWCO) from samples and attain excellent protein recovery with these centrifuge-based chromatography columns.

Manufacturer:  Thermo Scientific™ 87768

Catalog No. PI87768

Description

Thermo Scientific™ Zeba Spin Desalting Columns (40K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 5μL to 4mL samples.

These specially designed polypropylene centrifuge columns are pre-packed with high-performance Zeba Desalting Resin to facilitate rapid protein desalting with high recovery. The resin effectively traps salts and other small sample-components but does not impede proteins and other macromolecules, which pass directly into the centrifuge collection tube. These particular columns use Zeba Resin having a molecular-weight cutoff (MWCO) rating equal to 40K; this ensures that proteins larger than 40,000 MW will be recovered with high efficiency.

The easy-to-use spin column format dramatically improves results over standard drip-column methodologies. This system eliminates the need to wait for samples to emerge by gravity flow and to monitor fractions for protein recovery. Samples are processed in these desalting columns using a standard centrifuge or microcentrifuge. No chromatography hardware or column preparation steps are required. Multiple samples can be processed in less than ten minutes. Zeba Spin Desalting Columns are available in five different column sizes, and these provide trouble-free desalting and buffer exchange for sample volumes ranging from 5μL to 4mL.

Highlights:

• Fast centrifuge format – resin and columns are optimized for rapid processing by centrifugation with standard collection tubes and rotors

• Replaces drip-column methods – no need to collect and screen slowly-emerging protein fractions

• Exceptional protein yields – recover greater than 95% in typical applications with minimal dilution

• Five convenient sizes – columns with 75μL to 10mL of Zeba Resin provide desalting matched for sample volumes between 5μL and 4mL

• 40K MWCO – resin is rated to recover proteins larger than 40,000 daltons and retain (remove) solutes smaller than 2000 MW

Requires:

Microcentrifuge and microcentrifuge collection tubes (for 75μL and 0.5mL columns) or centrifuge and collection tubes (15mL tubes for 2 and 5mL columns; 50mL tubes for 10mL columns)

Specifications

• Column Type: Size-Exclusion, Proprietary Resin
• Cut Off or Exclusion Limit: 40 kDa
• Product Line: Zeba™
• Sufficient For: 5 samples, each 200 to 900uL

• Content And Storage: Upon receipt store at 4°C.
• Format: 15 mL conical, Spin Column
• Purification Target: Buffer Exchange, Protein