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Invitrogen™ Zika Virus Envelope (strain H/PF/2013) Polyclonal Antibody
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Catalog No. PIPA5143492
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100 μL
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PIPA5143492 100 μL
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Catalog No. PIPA5143492 Supplier Invitrogen™ Supplier No. PA5143492
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Description

Rabbit Polyclonal Antibody

Store as concentrated solution. Centrifuge briefly prior to opening vial. Positive control: Zika-infected Vero, Zika virus Envelope-transfected 293T, Zika virus Envelope-infected C6/36.

As a member of the Flaviviridae family, ZIKV has a single positive sense RNA genome, which is initially translated as a single polyprotein which is then cleaved post-transitionally into three structural proteins (C, PrM or M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5)(1). The glycosated NS1 forms a homodimer inside the cells and is necessary for viral replication and late in infection (2). NS1 is also secreted into the extracellular space as a hexameric lipoprotein particle, which assists the virus in immune evasion and pathogenesis by interacting with components from both innate and adaptive immune systems as well as other host factors (1). Reference 1) Song, H.; Qi, J.; Haywood, J.; Shi, Y.; Gao, G.F. (2016). Nat. Struct. Mol. Biol. 23, 456-458. 2) Suthar, M.S., Diamond, M.S. and Gale, M. Jr. (2013). Nat. Rev. Microbiol. 11, 115-128.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Zika Virus Envelope (strain H/PF/2013)
Applications ELISA, Western Blot, Immunocytochemistry
Classification Polyclonal
Concentration 1.14 mg/mL
Conjugate Unconjugated
Formulation PBS with 20% glycerol and 0.025% Proclin 300; pH 7
Gene Alias Zika Envelope
Host Species Rabbit
Immunogen Recombinant protein encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
Purification Method Antigen affinity chromatography
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Virus
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG
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