Invitrogen™ ZNF304 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA562207

Catalog No. PIPA562207

Description

Immunogen sequence: SLRQHQGDYD GQMLFSCGDE GKAFLDTFTL LDSQMTHAEV RPFRCLPCGN VFKEKS Highest antigen sequence identity to the following orthologs: Mouse - 43%, Rat - 43%.

ZNF304 acts as transcriptional regulator and plays a role in gene silencing. It probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of several tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. ZNF304 is also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) by inducing trimethylation of Lysine-27 of histone H3 (H3K27me3) in a Polycomb group (PcG) complexes-dependent manner. The protein associates at promoter regions of TSGs and mediates the recruitment of the corepressor complex containing the scaffolding protein TRIM28, methyltransferase DNMT1 and histone methyltransferase SETDB1 and/or the PcG complexes at those sites. It acts as a transcription factor involved in the metastatic cascade process by inducing cell migration and proliferation and gain resistance to anoikis of ovarian carcinoma (OC) cells via integrin-mediated signaling pathways. It associates with the ITGB1 promoter and positively regulates beta-1 integrin transcription expression. It promotes angiogenesis and tumor growth.

Specifications

• Antigen: ZNF304
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ZNF304
• Gene Alias: KRAB-containing zinc finger protein; zinc finger protein 304; ZNF304
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 57343
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q9HCX3
• Gene Symbols: ZNF304
• Immunogen: Recombinant protein corresponding to Human ZNF304. Recombinant protein control fragment (Product #RP-101284).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG