ZO-1 Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-2105	Rockland Immunochemicals 600401GU7	100 μg
50-199-2106	Rockland Immunochemicals 600401GU7S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against human ZO-1. Blast analysis of the sequence of the immunogen shows 100% identity with human.

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

Specifications

• Antigen: ZO-1
• Applications: ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human, Mouse
• Gene Accession No.: P39447, Q07157
• Gene ID (Entrez): 21872, 7082
• Primary or Secondary: Primary
• Product Type: Antibody

• Concentration: 1.05 mg/mL
• Classification: Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: BOS_20285; DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; Tjp1; ZO1; ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
• Gene Symbols: TJP1
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: TJP1