ZO-1 Rat anti-Canine, Mouse, Rat, Clone: R26.4C, eBioscience™ 

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2861	Invitrogen 14977680	25 μg
50-112-2862	Invitrogen 14977682	100 μg

Description

Description: The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine. ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain. ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer. ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C. ZO-1 is thought to anchor the actin cytoskeleton to the tight junction. ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression. Applications Reported: This R26.4C antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry. Applications Tested: This R26.4C antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay interest.

Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

Specifications

• Antigen: ZO-1
• Target Species: Canine, Mouse, Rat
• Isotype: IgG2a, kappa
• Monoclonal or Polyclonal: Monoclonal
• Format: Liquid
• Type: Antibody
• Storage Requirements: 4° C

• Clone: R26.4C
• Conjugate: Unconjugated
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Regulatory Status: RUO

For Research Use Only.