ZP-2, Rat anti-Mouse, Clone: IE-3, Millipore Sigma™

Rat Monoclonal Antibody

Supplier:  MilliporeSigma MABE114225UG

Catalog No. MABE114225

Description

Anti-ZP-2, clone IE-3, Cat. No. MABE1142, is a highly specific rat monoclonal antibody, that targets Zona pellucida sperm-binding protein 2 and has been tested in ELISA, Immunoprecipitation, Immunofluorescence and Western Blotting.

Zona pellucida sperm-binding protein 2 (UniProt: P20239; also known as Zona pellucida glycoprotein 2, Zp-2, Zona pellucida protein A) is encoded by the Zp2 (also known as Zp-2, Zpa) gene (Gene ID: 22787) in murine species. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The mammalian zona pellucida mediates species-specific sperm binding, induction of the acrosome reaction, and prevents post-fertilization polyspermy. ZP2 is expressed during the 2-week growth phase of oogenesis, prior to ovulation and may act as a secondary sperm receptor and is proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL and is exocytosed from cortical granules after fertilization, yielding an N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage plays an important role in the post-fertilization block to polyspermy. Female mice lacking Zp2 gene do not form a stable zona matrix and are sterile. (Ref.: Hinsch, KD and Hinsch, E. (1999). Andrologia.31(5):320-322.)

Specifications

• Antigen: ZP-2
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: Zp2;Zp-2;Zpa
• Immunogen: Purified mouse zonae pellucidae.
• Quantity: 25 μg
• Research Discipline: Epigenetics & Nuclear Function
• Test Specificity: Clone IE-3 detects Zona pellucida sperm-binding protein 2 in murine species.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG2a

• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
• Clone: IE-3
• Dilution: Immunoprecipitation Analysis: A representative lot detected ZP-2 in Immunoprecipitation applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800).Immunofluorescence Analysis: A representative lot detected ZP-2 in Immunofluorescence applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800).Immunofluorescence A representative lot detected ZP-2 in Immunofluorescence applications (Avella, M.A., et. al. (2016). Sci Transl Med. 8(336):336ra60).ELISA Analysis: A representative lot detected ZP-2 in Western Blotting applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800; Sun, W., et. al. (1999). Biol Reprod. 60(4):900-7).Western Blotting Analysis: A representative lot detected ZP-2 in ELISA applications (Sun, W., et. al. (1999). Biol Reprod. 60(4):900-7).
• Gene Alias: Zona pellucida sperm-binding protein 2;Zona pellucida glycoprotein 2;Zona pellucida protein A
• Host Species: Rat
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Form: Purified