Invitrogen™ ZRANB3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA597227

Catalog No. PIPA597227

Description

Immunogen sequence: ITKQQTKQNC TKRYITKEDV AVASMDKVKN VGGHVRLITK ESRPRDPFTK KLLEDGACVP FLNPYTVQAD LTVKPSTSKG YLQAVDNEGN PLCLRCQQPT CQTKQACKAN SWDSRFCSLK CQEEFWIRSN NSYLRAKVFE TEHGVCQLCN VNAQELFLRL RDAPKSQRKN LLYATWTSKL PLEQLNEMIR NPGEGHFWQV DHIKPVYGGG GQCSLDNLQT LCTVCHKERT ARQAKERSQV RRQSLASKHG SDITRFLVKK; Positive Samples: HeLa, Raji, Jurkat, 293T, Mouse liver, Mouse heart, Mouse brain, Rat liver; Cellular Location: Chromosome, Nucleus.

DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558). [UniProt].

Specifications

• Antigen: ZRANB3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ZRANB3
• Gene Alias: 4933425L19Rik; AH2; AI316834; Annealing helicase 2; C730006D09; DNA annealing helicase and endonuclease ZRANB3; DNA annealing helicase ZRANB3; Endonuclease ZRANB3; RGD1564952; Zinc finger Ran-binding domain-containing protein 3; zinc finger RANBP2-type containing 3; zinc finger, RAN-binding domain containing 3; ZRANB3
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 226409, 304761, 84083
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.57 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q5FWF4, Q6NZP1
• Gene Symbols: ZRANB3
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 818-1077 of human ZRANB3 (NP_0012734971).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG