Histology Reagents
Electron Microscopy Sciences Cobalt Naphthenate Solution 10 ML
Solution of 55% Cobalt Napthenate and 45% Mineral Spirit that is used as an activator for the Vestopal embedding resin.
AccuStandard, Inc Formaldehyde-DNPH | 1081-15-8 | MFCD00191364 | 1mL
Formaldehyde-DNPH | MW: 210.148 | 1081-15-8 | MFCD00191364 | 1mL
Electron Microscopy Sciences Araldite 507 450 ML
W.P.E. number is included on each bottle.
Specific Gravity: 1.14
CAS: 26447-14-3
Leica Biosystems Reagent Vessel With Handle for Autostainer XL, ST5010, ST5020
Reagent vessel with handle is a replacement vessel assembly designed to contain and transport staining reagents used in automated slide stainers. The assembly includes a lid and an ergonomic handle for safer handling and is sold individually for routine cytology and histology workflows.
- Replacement reagent vessel with handle and lid assembly.
- Compatible with Autostainer XL, ST5010, ST5020 (new style only).
- Sold individually for use as a replacement part.
- Intended for cytology and histology reagent containment.
Supply Solutions Sigma - Formalin Solution Neutral Buffered
Sigma - Formalin Solution Neutral Buffered
National Diagnostics HISTO-CLEAR II 5 GL
Histo-Clear II, Xylene substitute composed of ultra-refined light paraffin oil and orange oil. Greatly reduced citrus odor, 5 GL
Electron Microscopy Sciences Histo-Clear II, 1L
Histo-Clear II is a mixture of Aliphatic Hydrocarbon and Distilled essential oils that is food grade with a reduced citrus odor. It is an excellent non-toxic clearing agent that is replacing Xylene.
Electron Microscopy Sciences Glutaraldehyde Aqueous 50%, EM Grade 10 ML
OCHCH₂CH₂CH₂CHO F.W. 100.12
Aqueous glutaraldehyde, the universal fixative for electron microscopy, is purified to EM grade from commercial grade by "all-glass vacuum-distillation". It is the highest possible quality, free from polymers and other contaminents. Each lot is assayed prior to shipping assuring consistent purity and the highest possible quality.
If stored properly, our Glutaraldehyde has a very long shelf life. EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules sealed under dry nitrogen and 100 ml bottles that are kept refrigerated until it is shipped.
Grade: EM Grade
Electron Microscopy Sciences Bismuth Stain Kit 2
Bismuth specifically stains Golgi beads and interchromatin granules after fixation with glutaraldehyde, whereas it stains nucleoli, basic protein, and biogenic amines after formaldehyde fixation. Bismuth staining of sections is relatively nonspecific: it can stain glycogen, lysosomes, ribosomes, polysaccharides, ferritin, and DNA. M.A. Hayat, "Basic Techniques for TEM" p. 184 (1986). Enhancing Contrast in General (Riva, 1974). Kit contains 5x10 ml Solution A(Sodium tartrate in NaOH) 5x200mg Ingredient B (Bismuth subnitrate).
Visikol Inc Visikol HISTO 1 & 2 Combo (100 mL each)
Laboratory Reagent, Histological Reagent, Tissue Clearing Reagent, Visikol HISTO-1 and HISTO-2 Combo, 100mL each. A simple and rapid tissue clearing formula that can be used to image tissues in 3D without damaging the underlying cellular morphology. Designed to pair with immunolabeling and reversible so that 3D histology can be followed up with traditional 2D histology. The Visikol HISTO tissue clearing approach is comprised of two different reagents called Visikol HISTO-1 and Visikol HISTO-2 whereas Visikol HISTO-1 is used first and Visikol HISTO-2 is used second. However, for small tissues such as micro-tissues and thin brain slices (500 um), only Visikol HISTO-1 is required and tissues can be imaged in Visikol HISTO-1. Larger tissues must be placed in Visikol HISTO-2 to finish clearing and then these samples can be imaged in Visikol HISTO-2. WARNING: Do not use HISTO-2 within any polystyrene vessle.
Newcomer Supply Eosin Y Working Solution, 500 ml. Ready to use counterstain in the routine hematoxylin and eosin stain and can be used in either manual or automated staining platforms.
Use: Counterstain for H&E Stain. Newcomer Supply Eosin Y Working Solution is a ready to use counterstain in the routine hematoxylin and eosin stain and can be used in either manual or automated staining platforms. Eosin’s particular value is in its ability to distinguish between the cytoplasm of different types of cells by staining cytoplasmic components differing shades and intensities of pink to red. Shelf Life is 1 year from date of manufacture. Store at 15-30°C.
Electron Microscopy Sciences Formaldehyde/Zinc Fixative 4 LT
Prepared: Ready-to-use. Preserves fine nuclear details and good for immunostaining. Contains no mercury. Can be used with automated tissue processors. Works well with standard and special stains because zinc is a stain enhancing mordant.
Electron Microscopy Sciences Acetone: Xylene 500 ML
Used in Brown and Brenn Method for gram positive and gram negative Bacteria.
Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.
References are Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931) and AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222