Histology Reagents
Electron Microscopy Sciences Glutaraldehyde Aqueous 50%, EM Grade 10 ML
OCHCH₂CH₂CH₂CHO F.W. 100.12
Aqueous glutaraldehyde, the universal fixative for electron microscopy, is purified to EM grade from commercial grade by "all-glass vacuum-distillation". It is the highest possible quality, free from polymers and other contaminents. Each lot is assayed prior to shipping assuring consistent purity and the highest possible quality.
If stored properly, our Glutaraldehyde has a very long shelf life. EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules sealed under dry nitrogen and 100 ml bottles that are kept refrigerated until it is shipped.
Grade: EM Grade
Electron Microscopy Sciences Glutaraldehyde 25% Aqueous Solution, 111-30-8, MFCD00007025, 10 mL ampoule
Formula: OCHCH2CH2CH2CHO, MW: 100.12. After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde. 4 to (-18)°C.
Electron Microscopy Sciences Phloxine B Solution 0.5% 500 ML
Used in Thomas Method for Malarial Parasites, Thomas (1953). References: Thomas, J.T. Stain Techn., 28:311-312, 1953 and Luna, L.G. (ed.) Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, New York, P.129, c.1968
Electron Microscopy Sciences Bouin's Solution 20 ML
Bouin's solution can be used as a fixation and a staining fluid and is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. May be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
Electron Microscopy Sciences Histo-Clear II Non-toxic Clearing Agent 5 GL
Histo-Clear (Distilled essential oils –food grade) and Histo-Clear II (A mixtureof Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.
Electron Microscopy Sciences Bouin's Solution 30 ML
Bouin's solution can be used as a fixation and a staining fluid and is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. May be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
Electron Microscopy Sciences Gum Mastic 2.5% in Alcohol 500 ML
It is available for your convenience in a concentration of 2.5% in alcohol.
Electron Microscopy Sciences Bouin's Solution 10 ML
Bouin's solution can be used as a fixation and a staining fluid and is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. May be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.
Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.
Polysciences, Inc. Soft Nail, 250 mL
Made for very hard complex keratinized hair, nail and skin samples common in Mohs Histology laboratories. Hair, nail and skin samples that have been processed tend to flex and turn becoming difficult to cut and hold on slides for staining.
E K Industries Reagent Alcohol, 95%, 4 Gal Case
Melting point / freezing point ~-114 C, boiling range 78 C - 80 C, Flash point ~13 C
Electron Microscopy Sciences Gold Chloride Gold Chloride 0.1% Aqueous Solutions 500 ML
It is available for your convenience in a concentration of 0.1% aqueous.
Electron Microscopy Sciences Giemsa Working Solution 500 ML
Used in Wolbach's Giemsa Method Wolbach, Todd, and Palfrey (1922) Nuclei, collagen. References are Wolbach, S.B., Todd, J.L. and Palfrey, F.W., The Etilogy of Pathology Typhus, Harvard University Press, Cambridge, MA., p. 13 – 14,c 1922 and Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Edition, McGraw-Hill, Y, p. 119, c. 1968
Electron Microscopy Sciences Thionin Solution, 0.5% in 20% Alcohol 500 ML
It is available for your convenience in a concentration of 0.5% in 20% alcohol.
Electron Microscopy Sciences Acetone: Xylene 500 ML
Used in Brown and Brenn Method for gram positive and gram negative Bacteria.
Also see the Taylor Modification of the Brown and Brenn +/- technique noted for the varying differentiation available. Over-differentiation in the B&B sep 6 is a problem with some sections; run the control slides at varying rates to determine the amount for the specific organism.
References are Brown J.H. and Brenn, L. Bull. Johns Hopkins Hosp., 48:69 (1931) and AFIP Manual of Histologic Staining Techniques: 3rd. ed., ed. G. Luna; New York: McGraw-Hill Publications, c. 1968, p. 222
Electron Microscopy Sciences Acid-Alcohol 1% 500 ML
Used in McManus' Method (PAS) for Glycogen, McManus (1948). McManus, JFA: Stain Tech.; 23:99 (1948), AFIP Manual of Histologic Staining Techniques; L.G. Luna, 3rd Ed., New York: McGraw-Hill Publications, c. 1968, p. 160, or Mowry, RW: Annals of the New York Academy of Science, 106:402 (1963).