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A sphingolipid; levels are increased by PPMP or MAPP in isolated human neutrophils and are correlated with an increase in apoptosis; accumulates in the lungs of patients with cystic fibrosis compared with the lungs of patients with pulmonary hypertension; plasma levels are increased in patients with acute coronary syndrome, and an increased plasma ratio of C16 to C24:0 ceramide is associated with major adverse cardiac events; levels are increased in tumor tissue from patients with breast cancer and is associated with metastatic cancer
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C12 NBD ceramide (d18:1/12:0) is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity.{14063} It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C12 NBD ceramide (d18:1/12:0). Acid ceramidase does not appear to utilize C12 NBD ceramide (d18:1/12:0) as a substrate. The possibility exists forC12 NBD ceramide (d18:1/12:0) to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity.
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A naturally occurring sphingolipid; levels are elevated in the prefrontal cortex and cerebellum in a rat model of STZ-induced diabetes, as well as in the liver in mouse models of Niemann-Pick type C1 disease; plasma are decreased in patients with prostate cancer; has been used in model membranes with POPC and cholesterol in the study of lipid raft dynamics and membrane organization
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