Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1X PBS, Dulbecco's Solution (with Calcium and Magnesium). 1000mL, Sterile 2 pack.
137mM Sodium chloride, 0.5mM Magnesium chloride, 8.1mM Disodium phosphate, 0.9mM Calcium chloride, 1.47mM Monopotassium phosphate, 2.7mM Potassium chloride
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS - 20L-CBY
Dulbecco s Phosphate Buffered Saline DPBS - 20L-CBY (For Research Use and Further Manufacturing Only)
Teknova 1X PBS Solution, pH 7.4, 1000mL, Sterile. 2 Pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Teknova 10X PBS Solution, pH 6.0. 1000 mL, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
INNOVAPREP LLC Elution Fluid Can, Tris-buffered solution, Water >99.9%, Tween 20 0.075%, Carbon Dioxide propellant, 125 psi pressure, 50 extractions per can, Non-flammable gas
The InnovaPrep HC08001 Elution Fluid Can is a specially formulated Tris-buffered solution designed for efficient recovery of particles using the InnovaPrep Concentrating Pipette systems. This carbonated elution fluid employs patented Wet Foam Elution™ technology for high recovery rates of microorganisms and particles, maintaining their integrity for various analytical applications. The pressurized can format enhances laboratory workflows with its ready-to-use capability, facilitating rapid and reliable sample concentration.
- Optimized particle recovery through Wet Foam Elution™ technology
- Preserves integrity for downstream compatibility
- Supports up to 50 extractions per can for high throughput
- Pressurized can ensures safe and immediate use
Teknova 10X PBS Solution, 4L, Non-Sterile. 4 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Electron Microscopy Sciences Phosphate Buffered Saline, 10X 1 GL
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Pyridoxal which is an aldehyde has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
Boston Bioproducts Inc Saponin Cell Permeating Solution (0.5% w/v in PBS) - 4x100ml
Saponin Cell Permeating Solution (0 5% w/v in PBS) - 4x100ml (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
