Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences TrizmaR base Primary Stand25KG
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations such as Tris-EDTA (TE) buffer Tris-acetate-EDTA (TAE) buffer Tris-borate-EDTA (TBE) buffer etc. It is pure essentially stable relatively non-hygroscopic and has a high equivalent weight.
Boston Bioproducts Inc PIPES Buffer (1 M, pH 6.8) - 500ml
PIPES Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 500ml
HEPES Buffer (1 M pH 7 6 pH Adjusted with KOH) - 500ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences MES hemisodium salt 98 tit100G
SODIUM MES hemisodium salt 98 tit100G
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4041909 BETA TRI CALCIUM PHOSPHTE 100G
Apexbio Technology LLC Annexin V-Cy5/SYTOX Green Apoptosis Kit 20assays
Annexin V is a Ca2 dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS resides on the inner surface of the cell membrane s phospholipid bilayer During early stages of apoptosis it translocates from the inner leaflet to the outer surface thus exposing itself to the extracellular environment Cy5-labeled Annexin V can serve as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy within 10-20 minutes SYTOX Green is a non-cell-permeable cyanine fluorescent dye and stains only dead cells It can be used effectively to differentiate normal viable cells from necrotic cells For cells at late apoptotic stages or dead cells SYTOX Green can penetrate the disordered regions of cell membrane reach the cell nucleus embedding into the DNA double helix to form a SYTOX Green-DNA complex resulting in a 500-fold increase of fluorescence intensity with bright green fluorescence
Alkali Scientific CellPro™ DPBS 10X, 500ml, 10/Case
Balanced salt solution used for multiple cell culture applications, (liquid form). Certificate of Analysis available electronically. Standard stock items from 100 mL to 200 L Volumes up to 1000 L available as made to order Not for diagnostic or therapeutic use. For making isotonic solutions for cell or tissue rinsing, cell dilution, reagent prep, transportation etc. where maintenance of cell integrity is required for further manufacturing or research use.
Alkali Scientific TE Buffer, 1X, 500 Milliliters
TE Buffer, 1X, 500 Milliliters is a pre-made solution used for a variety of molecular biology applications that require a stable pH and buffering capacity. This formulation, specifically designed for use in nucleic acid purification, electrophoresis, and other biochemical assays, ensures that DNA and RNA samples are preserved and stabilized during handling. The 1X concentration is ready for immediate use, eliminating the need for dilution. It maintains an optimal pH and ionic strength to protect sensitive molecules from degradation, making it an essential component in any laboratory focusing on nucleic acid research and molecular diagnostics.
ILEX LIFE SCIENCES LLC PAN-Biotech Dulbecco's Phosphate-Buffered Saline (DPBS), w: Ca and Mg (6 x 500 ml)
DPBS, WITH: Ca and Mg. QUANTITY: 6 x 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: Dulbecco's Phosphate-Buffered Saline (DPBS) with calcium chloride and magnesium chloride. The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions. These solutions are usually used for washing tissues and cells. Often they are combined with other agents in cell and tissue treatment.
AG Scientific Inc Phosphate Buffered Saline 1 L
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.Ready to use sterile 1X solution, pH: 7.25 - 7.55