Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 8.3. 500mL, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Hampton Research Tris Buffer
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL) Please note items from this supplier are non-returnable.
National Diagnostics Tris-Glycine Electroblotting Buffer (10X) 4 Liter
Tris-Glycine Electroblotting Buffer (10X) is a concentrated solution in distilled/deionized water. This buffer is intended to act as the tank buffer in Western electroblotting procedures. It is also the recommended buffer for use with nitrocellulose and nylon membranes.Tris-Glycine Electroblotting Buffer (10X) is already in solution which eliminates the weighing, mixing and adjusting of the pH necessary with powdered buffers. To prepare 1 liter of working strength buffer, add 100 ml of Tris-Glycine Electroblotting Buffer to 200 ml of methanol and 700 ml of distilled/deionized water.Tris-Glycine Electroblotting Buffer (10X) contains 0.25M Tris base and 1.92M glycine, with a pH of 8.4.
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 500g
Linear Formula NH2C(CH2OH)3, ≥99.9% (titration), Molecular Weight 121.14, bp 219-220 °C/10 mmHg(lit.), mp 167-172 °C (lit.), Primary Standard and Buffer, crystalline, Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Thermo Scientific Production Chemicals and Services 10MM TRIS-HCL 1MM EDTA
NC3588246 10MM TRIS-HCL 1MM EDTA
Thermo Scientific Production Chemicals and Services 50MM TRIS 10MM EDTA PH 7.2
NC3588255 50MM TRIS 10MM EDTA PH 7.2
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | 1185-53-1 | MFCD00012590 | 1KG
Trizma(R) hydrochloride BioPerformance Certified, suitable for cell culture, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 157.6 | 1185-53-1 | MFCD00012590 | 1KG
Bioworld TBE Buffer, Quick-Mix Pack (25X), 2 PK(s)
Product DescriptionTBE (Tris-Borate-EDTA) quick-mix pack prepares 1L of 25X concentrate. Each pack contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 1kg
BIS-TRIS | Purity: ≥98.0% (titration) | MW:209.24 | 6976-37-0 | MFCD00002853 | 1kg