Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 8.0 Solution. 4L, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld Tricine-SDS Sample Buffer, Non-Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under non-reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, and 0.01% phenol red.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
![Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B75000-100.0.JPG-250.jpg)
Research Products International Corp bis-TRIS [bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane], 100 Grams
Substance Name: bis-(2-hydroxyethyl)aminotris(hydroxymethyl)methane
CAS Number: 6976-37-0
Molecular Formula: C8H19NO5
Molecular Weight:?209
Chemical Purity:?>99%
Soluble in: Water
Store at:?Room Temperature
Quality Biological Inc Tris HCl (2 M) pH 8.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
Quality Biological Inc TE pH 8.0, 1000 mL
TE is composed of buffering agent Tris, and chelating agent EDTA. ¿ EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). ¿ Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. ¿ Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immunohistochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS hydrochloride PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture | Mol Wt: 245.7 | 124763-51-5 | MFCD00171252 |
BIS-TRIS hydrochloride PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture | 124763-51-5 | MFCD00171252 |
LAB BIOREAGENTS NGB I Buffer 100ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
Medchemexpress LLC Tris buffered saline | 10 TABLETS
Tris buffered saline is supplied as ready-to-dissolve tablets for preparing Tris/NaCl buffer solutions commonly used in molecular biology and protein applications. The tablet format simplifies buffer preparation and storage while delivering a stable pH environment for assays and electrophoresis.
- Ready-to-use tablet format for convenient buffer preparation.
- One tablet dissolves in 15 mL deionized water to yield 0.05 M Tris and 0.15 M sodium chloride.
- pH approximately 7.6 at 25 °C.
- White to off-white solid tablets for easy visual quality check.
- Suitable for DNA/RNA extraction, PCR, and protein electrophoresis.
- Compact packaging reduces storage space and eases transport.
Kd Medical Inc TRIS BUFF SALINE,10X PH 7.6 1L
Commonly used buffer used as a wash buffer in multiple laboratory applications. 10X concentrated TBS is ready to be diluted to a 1X working solution. pH 7.6, one 1 Liter bottle.
Kd Medical Inc TRIS 1M, PH 8.5, 1000ML BTL
Buffer used in a wide range of Molecular Biology applications. 1 Molar solution pH 8.5. One 1 Liter bottle.
Sigma Aldrich Fine Chemicals Biosciences Edetic Acid | 60-00-4 | MFCD00003541 | 200mg
Edetic Acid | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 200mg
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G