Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 1227502-35-3 | 5-Bromo-3-(hydroxymethyl)pyridin-2(1H)-one | MFCD30364462 | 250mg
ChemScene | 5-Bromo-4-fluoro-N-methyl-2-nitroaniline | 100mg | 569145939 | CS-0102151 | 1356483-97-0 | MFCD30073170 | 249.039 | C7H6BrFN2O2
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Kd Medical Inc TRIS BUFFER, 2M, PH 7.5, 500ML
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. Tris Solution, 2M, provides a convenient stock solution for preparing Tris buffers> One 500 ml bottle.
Bioworld Tricine-SDS Sample Buffer, Non-Reducing (2X), 25 mL
Product DescriptionTricine-SDS is a sample buffer under non-reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, and 0.01% phenol red.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
2M Tris,3M Sodium Chloride,1.0% Tween-20
Hampton Research HR2-579/1.0 M TRIS hydrochloride - 100 ml
HR2-579/1.0 M TRIS hydrochloride - 100 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences Piperazine BioUltra, anhydrous, >=99.0% (T) | 110-85-0 | MFCD00005953 | 25G
Piperazine BioUltra, anhydrous, >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 25G
Bioworld Tris-Glycine Transfer Buffer (10X), 1 L
Tris-Glycine Transfer Buffer, 10X Concentrate.For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.To make 1X Transfer Buffer from 10X concentrate: Mix 100 ml of 10X Transfer Buffer, 200 ml of methanol and 700 ml of dd water per liter.Foreign activity: Protease, none detected Storage temp.: 2-8°C
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml.
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8