Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Bioworld Tris Buffered Saline Packs, 10 PK(s)
Tris Bufferd Saline Packs An ideal all-purpose binding buffer.Each pack yields 500 ml of 25 mM Tris, 0.15 M NaCl, pH 7.2 when dissolved in 500 ml deionized water (total 5 liters). Quantity: 10 packs
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Medchemexpress LLC P62-mediated mitopha 10mM 1mL | 1809031-84-2 | 392.15 g·mol⁻1 | C14H9IN4O2 | 1 ML
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P62-mediated mitophagy inducer is a small-molecule research reagent that activates p62-mediated mitophagy without recruiting Parkin or collapsing mitochondrial membrane potential. It is supplied as a 10 mM stock solution (1 mL) for use in cellular and biochemical studies of selective mitochondrial clearance and autophagy pathways.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Provided as a ready-to-use 10 mM stock solution in 1 mL.
- Suitable for cellular and biochemical assays investigating mitophagy and autophagy.
- Stable storage recommendations: powder at -20°C for up to 3 years; in solvent at -80°C or -20°C as indicated.
- Comes with chemical identification and characterization data (CAS, formula, molecular weight) in the supplier datasheet.
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S2 Media 20x Tris Buffered Saline (400mM Tris-HCl, 3M NaCl), pH 7.4, Sterile, 1L Media Bottle, 1ea
Tris Buffered Saline (TBS), formulated at a 20x concentration, is used in cell culture, molecular biology, protein research, and purification applications. It can be utilized as a wash buffer, storage buffer, and for resuspension of cells. he Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration.• Tris Buffered Saline should be used by trained professionals within a laboratory setting. • Tris is an abbreviation for tris(hydroxymethyl)aminomethane).• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• For Research Use Only (RUO). Not for use in diagnostic procedures.
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Biotang Inc MOPS-Buffer 0.2M, pH 8.5, 100 ml
MOPS-Buffer 0.2M, pH 8.5, 100 ml.
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eMolecules benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g
Pharmablock | benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g | 551109237 | PBJP030 | 305.374 | C17H23NO4
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Strem, An Ascensus Company CAS# 60-00-4. 500g. Ethylenediaminetetraacetic acid, 99+%. MFCD00003541
CAS# 60-00-4. 500g. Ethylenediaminetetraacetic acid, 99+%. MFCD00003541. Molecular Weight: 292.24. Molecular Formula: (HO2CCH2)2NCH2CH2N(CH2CO2H)2. Color/form: white xtl. Strem# 07-0573. http://www.strem.com/catalog/v/07-0573/
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Medchemexpress LLC P62-mediated mitopha 100mg | 1809031-84-2 | 392.15 | C14H9IN4O2 | 100 MG
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P62-mediated mitophagy inducer (PMI) is a small-molecule activator of p62-mediated mitophagy used as a pharmacological tool to study mitophagy pathways. It activates mitochondrial autophagy without recruiting Parkin or collapsing mitochondrial membrane potential, and it stabilizes Nrf2 and upregulates p62 at active concentrations.
- Activates mitochondrial autophagy independent of Parkin recruitment.
- Remains active in cells lacking a fully functional PINK1/Parkin pathway.
- Stabilizes Nrf2 and upregulates p62 expression at effective concentrations.
- Well characterized by NMR, HRMS, and elemental analysis for research applications.
- Solid compound, soluble in DMSO for stock preparation.
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Bioworld Tris Buffered Saline, 1 L
Tris Buffered SalineContains: 0.1M Tris and 0.3M NaCl, pH 7.5
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Teknova 1M Tris-HCl, pH 8.0, 0.5mL, 50 Tubes per Pack, Sterile. 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
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Teknova 1M Tris-HCl, pH 7.5. 500mL, Sterile, DNAse/RNAse Tested 8 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
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Biotang Inc TBE Sample Buffer (6X), 25 ml
TBE Sample Buffer (6X), 25 ml.
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eMolecules 666747-04-2 | 3-Bromo-4-(hydroxymethyl)phenol | MFCD18391433 | 250mg
Ambeed | 3-Bromo-4-(hydroxymethyl)phenol | 250mg | 552587784 | A149354 | 666747-04-2 | MFCD18391433 | 203.035 | C7H7BrO2
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Cambridge Isotope Laboratories CDC BFR PAR Solution (unlabeled) 1000 ng/mL in nonane 1 2 mL
CDC BFR PAR Solution (unlabeled) 1000 ng/mL in nonane 1 2 mL
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Cayman Chemical Tris Buffer Concentrate (10X), 10 mL
Tris Buffer Concentrate (10X), 10 mL
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Teknova 1M Tris-HCl pH 8.5 Solution
1M Tris-HCl, pH 8.5 Solution, 20L Bag, Sterile. 4 Pack.
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