Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 6X1L
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 6X1L
Sigma Aldrich Fine Chemicals Biosciences Glycine Buffer Solution | 100ml
Glycine is used as a bulking agent buffers. Glycine at low concentrations prevents pH decrease in solutions. It also stabilizes a protein when present in an amorphous state.
Research Products International Corp PBS 1X SOLN 1ML TUBES 36/CS
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 1.0 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 4.5 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 1.0 ml of PBS per tube
Sterile
36 x 1.0 ml
Promocell GmbH PromoCell Dulbeccos PBS 500 ml, w/o Ca++/ Mg++
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.Dulbecco's PBS can be purchased with or without Ca^2 ^/ Mg^2 ^.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
Suitable for growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Sigma Aldrich Fine Chemicals Biosciences PIPES 99 titration1KG
PIPES is a member of the ethanesulfonic acid buffer series first introduced by Good et al. developed to meet certain criteria midrange pKa maximum water solubility and minimum solubility in all other solvents minimal salt effects minimal change in pKa with temperature chemically and enzymatically stable minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 C is near physiological pH PIPES has applications in cell culture work.
Sigma Aldrich Fine Chemicals Biosciences BES 99.0 titration250G
BES (N N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES a sulfonic acid-containing cross-linking agent induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
Sigma Aldrich Fine Chemicals Biosciences TRIZMA BASE ANHYDROUS 99.9%
Trizma® baseanhydrous, free-flowing, Redi-Dri™, ≥99.9%cas 77-86-1