Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 9.2. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Biotang Inc Phosphate-buffered saline (PBS, 10X), 2000ml
Phosphate-buffered saline (PBS, 10X), 2000ml.
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
HIMEDIA Dulbeccos Phosphate Buffered
Dulbecco s Phosphate Buffered Saline 1X w/o Phenol red Calcium and Magnesium
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi1L
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Alkali Scientific QuickSilver™ Buffer Powder, Tris-MOPS-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Dulbecco's Phosphate Buffered Saline With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 500ML
Teknova 50mM Tris, 150mM Sodium Chloride. pH 7.5, 1000mL. Endotoxin Tested. Sterile.
50mM Tris, 150mM Sodium Chloride. pH 7.5, 1000mL. Endotoxin Tested. Sterile.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G
Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) base >=99.0% (T) | 77-86-1 | MFCD00004679 | 100G
Trizma(R) base >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G