Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 25 G
MOPS sodium salt is a buffering agent utilized in biological applications. MOPS buffer is effective in maintaining the pH of mammalian cell culture media and has been shown to significantly improve the thermal stability of BSA. It is relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Buffering agent for biological research.
- Maintains pH in mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Can be used in the preparation of keratinocyte and microbial culture media.
Medchemexpress LLC MES hydrate | 1266615-59-1 | MFCD00149409 | 99.9% | 10 G
MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature, a factor to consider when used at varying temperatures. MES hydrate exhibits good buffering properties in biological experiments and has a poor affinity for metal ions, making it suitable for studies involving such ions.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at approximately pH 6.15
- Poor affinity with metal ions
Medchemexpress LLC 1,4-Piperazinedipropanesulfonic acid, β1,4-dihydroxy- | 68189-43-5 | ≥95.0% | 25 G
POPSO is a zwitterionic buffer that increases osmolality and demonstrates significant inhibition of anion uniport. It inhibits chloride uniport with an IC50 value of 24 mM. POPSO also enhances copper uptake and toxicity in alga and impairs the mitochondrial inner membrane. The working pH range for POPSO sesquisodium salt is 7.2-8.5.
- Zwitterionic buffer
- Increases osmolality
- Marked inhibition of anion uniport
- Inhibits chloride uniport with an IC50 value of 24 mM
- Enhances copper uptake and toxicity in alga
- Impairs mitochondrial inner membrane
- Working pH range (for sesquisodium salt): 7.2-8.5
Teknova 20X PBS Solution with 0.1% Tween-20. 1000 mL, Non-Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific CellPro™ Tris Buffered Saline 20X, pH 7.4, 20L (modified)
Alkali Scientific™ CellPro™ TBS, 20X, pH 7.4, offers a sterile and concentrated Tris-buffered saline (TBS) solution, meticulously formulated to meet the demands of various molecular biology applications. With a pH of 7.4, this buffer provides optimal conditions for a wide range of biochemical and immunological assays, ensuring reliable and reproducible results. The 20X concentration allows for convenient dilution to the desired working strength, providing flexibility and efficiency in the laboratory. Sterilized to maintain purity and eliminate the risk of contamination, CellPro™ TBS is ready for immediate use in your research experiments. Trust in the quality and consistency of CellPro™ buffers to support your molecular biology endeavors, delivering accurate and precise results every time.
Crescent Chemical Co Inc TBE BUFFER (10X)
TBE Buffer (10x) 10 x concentrated aqueous solution.TBE Buffer is widely used for the electrophoresis of nucleic acids and has a higher buffer capacity than TAE. It can be used for DNA and RNA polyacrylamide and agarose gel electrophoresis. Size - 2x500ML
Crescent Chemical Co Inc TE BUFFER (100X)
TE Buffer (100X) 100 x concentrated aqueous solution. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42554.01
Research Products International Corp Bicine Ultra-Pure, 250 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Crescent Chemical Co Inc TRIS(HYDROXYMETHYL)AMINOMETHAN
Tris(hydroxymethyl)aminomethane.hydrochloride molecular biology grade DNase/RNase Protease not detected. Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 39787.01 CAS 1185-53-1
Teknova 1M Tris-HCl, pH 7.4. 100mL. Sterile 10 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.