Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 502650-56-8 | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | 502650-56-8 | 172.224 | C9H16O3
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Gold Biotechnology Inc Tris HCl 500 g
Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml.
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Buffer; National Diagnostics; Tris-Glycine-SDS PAGE (10X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Size: 1L
Bioworld Tris-Acetate-SDS Running Buffer (10X), 500 mL
TRIS-Acetate-SDS Running Buffer (10X) All bioWORLD running buffers are prepared with high quality reagents. Technical Information:Dilute with deionized water to make 1X. 10X concentration1X contains: 50mM Tris base, 50mM Tricine, 3.5mM SDS pH 8.24
Bioworld Tris-Glycine Sample Buffer (4X), 50 mL
Product DescriptionTris-Glycine (TG) sample buffer has a concentration of 4X. It contains 400 mM Tris-HCl at a pH of 8.6, 40% glycerol, and 0.02% bromophenol blue.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Teknova 5M Sodium Chloride Solution, 200L Bag
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Alkali Scientific Saline 0.85% 20mL, 10/Pk
Saline 0.85% is a sterile isotonic sodium chloride solution widely used in microbiology and laboratory testing. The concentration closely matches the osmotic pressure of bacterial and mammalian cells, helping maintain cellular integrity during handling and dilution. Unlike distilled water, which can cause cells to burst or shrink, this balanced saline preserves cell viability and structure. Because the solution contains no nutrients or buffering components, it provides an inert environment that allows microorganisms to remain stable without stimulating growth. The 20mL tube format offers a convenient volume for washing microbial cells, preparing suspensions, or diluting cultures prior to analysis in microbiological testing and quality control procedures.
Alkali Scientific Saline 0.45% 2mL, 10/Pk
Saline 0.45% is a sterile sodium chloride solution commonly referred to as half normal saline. With a salt concentration lower than physiological saline, it is considered hypotonic relative to most biological cells. This property allows water to move into cells through osmosis, causing them to swell under controlled laboratory conditions. Such behavior is useful in certain cytological and cell preparation procedures where swelling assists with cell lysis or chromosome spreading for microscopic examination. The solution is also used for rehydrating freeze dried reagents, enzymes, or antibiotics when lower ionic strength is required. In laboratory workflows it may also serve as a gentle washing or dilution solution in molecular biology and biochemical procedures.
Med Vet International Irrigation Solution Saljet 0.9% Sodium Chloride, Preservative Free Not for Injection Unit Dose Vial 30 mL, 64938-009-01, 240/Case
0.9% sodium chloride weight by volume Each 30-mL vial has a twist-off top 40 vials per box
Carterra 10x HBS no Tween - 1 Liter
10x HBS (no Tween) - 1 Liter
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eMolecules 1036262-54-0 | trans-2,4-Bis-hydroxymethyl-azetidine-1-carboxylic acid tert-butyl ester | J & W PharmLab LLC434.530 | C20H38N2O8 | 96.000 | CC(C)(C)OC(=O)N1[C@@H](CO)C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067
trans-2,4-Bis-hydroxymethyl-azetidine-1-carboxylic acid tert-butyl ester | J & W PharmLab LLC | 1036262-54-0434.530 | C20H38N2O8 | 96.000 | CC(C)(C)OC(=O)N1[C@@H](CO)C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067