Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Research Products International Corp Phosphate Buffered Saline, 10X Solution, 500 Milliliters
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
![Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10900-36.0.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 1X Solution, 4.5ml Pre-Filled Tubes, Sterile, 36 Tubes per Case,10ml Tube Size
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 4.5 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 10 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 4.5ml of PBS per tube
Sterile
36 x 4.5 ml
Andwin Scientific VWR PBS-T BUFFER POWDER 100/CS
VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.Buffer powder quickly dissolves to make 1L of 1X solutionNo weighing or measuring of separate componentsNo pH adjustments requiredEconomical shipping, long shelf lifeQuality and consistency guaranteedVWR® Pre-measured buffer packs are extremely easy to use and are formulated so each pack makes 1 Liter of 1X buffer. Simply tear open a pack and mix completely with 1 liter of distilled water.Shipping costs are also minimal, as each case of 50 or 100 packs is compact and weighs only 2.5 Lbs.
Alkali Scientific CellPro™ PBS, .05% Tween 20, 1X, pH 7.4, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Electron Microscopy Sciences Phosphate Buffered Saline, 10X 1 GL
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
Med Vet International Zoetis Albon, 125mg, 200ct.
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
125mg Albon (sulfadimethoxine) from Zoetis effectively treats a broad range of bacterial infections commonly found in dogs and cats. This product is indicated for use in dogs and cats with respiratory, genitourinary tract, and soft-tissue infections.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics physiological osmolarity and ion concentrations, making it ideal for applications including protein purification, Western blotting and immunoassays, sample dilution, and as a transport medium. Packaged as a 10X concentration, this solution can be diluted to support its application.
Cell Signaling Technology Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml
Teknova 20x PBS with 0.05% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Apexbio Technology LLC Annexin V-APC/7-AAD Apoptosis Kit 20tests
Annexin V-APC/7-AAD Apoptosis Kit is possible to differentiate cells at various stages of apoptosis and distinguish dead cells Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit contains the nuclear dye 7-AAD a fluorescent probe that specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot permeate intact cell membranes However cells in the late apoptotic or necrotic stages exhibit compromised membrane integrity allowing 7-AAD to enter and stain the nuclei
Abcam PBS Tablets (1000 ml), 100TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Alkali Scientific CellPro™ PBS 10X, pH 7.2, without calcium, magnesium, 500mL, 500ml, 10/case
CellPro™ PBS, 10X, pH7.2, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Revvity Health Sciences Inc DELFIA L*R wash concentrate (25x) Standard, 250 mL
DELFIA L*R wash concentrate (25x) Standard, 250 mL