Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 5kg
Ethylenediaminetetraacetic acid | Purity: ≥98.5%, powder | MW:292.24 | 60-00-4 | MFCD00003541 | 5kg
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, anhydrous | 60-00-4 | MFCD00003541 | 500g
Ethylenediaminetetraacetic acid BioUltra, anhydrous | Purity: 99% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate (Standard) | 145224-94-8 | 99.95% | 50 MG
MES monohydrate (Standard) is the analytical standard of MES (monohydrate), intended for research and analytical applications. MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments.
- Used for research and analytical applications.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS.
Medchemexpress LLC Bicine | 150-25-4 | 98.0% | 10 G
Bicine is a buffer utilized in physiological research. It is a derivative of the simple amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection. This product appears as a white to off-white solid.
- Buffer for physiological research
- Derivative of amino acid glycine
- Enhances sensitivity of guanosine enzyme histochemical detection
- White to off-white solid appearance
- Molecular weight: 163.17
- Chemical formula: C6H13NO4
- Shipped at room temperature
- Stable at room temperature for 3 years
- Stable in solvent at -80°C for 2 years or -20°C for 1 year
- For research use only
Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, particularly in the preparation of enzyme buffers.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Especially useful in the preparation of enzyme buffers
Medchemexpress LLC 1,4-Piperazinedipropanesulfonic acid, β1,4-dihydroxy- | 68189-43-5 | ≥95.0% | 25 G
POPSO is a zwitterionic buffer that increases osmolality and demonstrates significant inhibition of anion uniport. It inhibits chloride uniport with an IC50 value of 24 mM. POPSO also enhances copper uptake and toxicity in alga and impairs the mitochondrial inner membrane. The working pH range for POPSO sesquisodium salt is 7.2-8.5.
- Zwitterionic buffer
- Increases osmolality
- Marked inhibition of anion uniport
- Inhibits chloride uniport with an IC50 value of 24 mM
- Enhances copper uptake and toxicity in alga
- Impairs mitochondrial inner membrane
- Working pH range (for sesquisodium salt): 7.2-8.5
![Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660370.jpg-250.jpg)
Alkali Scientific HEPPS [N (2 Hydroxyethyl)piperazine N' (3 propane sulfonic acid)] [EPPS], 100 G
HEPPS (N-(2-Hydroxyethyl)piperazine-N'-(3-propane sulfonic acid)) is a biochemical reagent available in a 100g bottle. This buffering agent is commonly used in laboratory settings to maintain a stable pH in aqueous solutions, particularly in biochemistry and molecular biology experiments. HEPPS is frequently used for enzyme assays, protein purification, and cell culture applications where precise pH control is necessary. It has a stable buffering capacity and works well at physiological pH levels, making it ideal for maintaining pH balance in a variety of biological assays. This reagent is essential for accurate experimental results in numerous biochemical processes.
Teknova qPCR DNase Buffer. 100mL, Sterile. 10 pack.
10.31mM Magnesium Chloride,10.31mM Calcium Chloride,10.31mM Tris-HCl
C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502147855.JPG-250.jpg)
eMolecules 1036262-54-0 | trans-2,4-Bis-hydroxymethyl-azetidine-1-carboxylic acid tert-butyl ester | J & W PharmLab LLC434.530 | C20H38N2O8 | 96.000 | CC(C)(C)OC(=O)N1[C@@H](CO)C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067
trans-2,4-Bis-hydroxymethyl-azetidine-1-carboxylic acid tert-butyl ester | J & W PharmLab LLC | 1036262-54-0434.530 | C20H38N2O8 | 96.000 | CC(C)(C)OC(=O)N1[C@@H](CO)C[C@@H]1CO.CC(C)(C)OC(=O)N1[C@H](CO)C[C@H]1CO | 1g | 534135067
Research Products International Corp Tris Glycine Buffer 10x Powdr
Dry homogeneous blend of molecular biology grade TRIS base and Glycine. Add 174.4g to make 1L of 10x Concentrate.