Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotium Blocking Buffers Background Reducers Fluorescence Microscopy Chemical Reagents TrueBlack 1/EA
This buffer is a component of the TrueBlack Background Suppressor System (Permeabilizing) It is an excellent general use non-mammalian blocking buffer with detergent for intracellular immunofluorescence Not recommended for use with HRP (contains azide) 100 mL bottle
Abcam PBS Tablets (1000 ml), 100TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences PBS (endotoxin-free) (sterile) (10ml)
Sterile and endotoxin-free PBS for use with TLRGRADE™ or endotoxin-free grade reagents. Product has been subjected to multiple rounds of LPS removal by adsorption with activated charcoal. Long Term Storage: +4°C.
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 20L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Miltenyi Biotec, Inc. CliniMACS PBS/EDTA Buffer
Suitable for cell processing and cell separation in combination with the CliniMACS System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
Miltenyi Biotec, Inc. CliniMACS PBS/EDTA Buffer 3L (CR) US
Suitable for cell processing and cell separation in combination with the CliniMACS System.
This product is subject to the following: Miltenyi Biotec, Inc. Terms and Conditions of Sale For Products and Services
AG Scientific Inc Hygromycin B in PBS, 10X2 ML
The concentration of Hygromycin B in PBS buffer is 50 mg/ml. Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells. Hygromycin's longstanding application as a gene selection antibiotic, has a powerful new role in genome editing utilizing the CRISPR/CAS9 system.CAS Number: 31282-04-09Molecular Weight: 527.53Chemical Formula: C20H37N3O13Solubility: Soluble in WaterStorage Temperature: 2-8CResearch or further manufacturing use only, not for food or drug use.
AdipoGen PBS Endotoxin-free (sterile), 10 mL
Synonyms: Phosphate Buffer Saline Endotoxin-free (sterile). Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
Rockland Immunochemicals Normal Saline - MB-049-1000
This solution is commonly referred to as physiological saline or isotonic saline.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Crude cell extracts contain a variety of endogenous enzymes capable of degrading proteins such as phosphatases and proteases The optimal method to enhance the yield of intact proteins is to supplement with inhibitors targeting these known enzymes This Protease Inhibitor Cocktail is used in plant cell and tissue extracts to improve protein stability The cocktail inhibits proteases that degrade both non-phosphorylated and phosphorylated protein substrates The Protease Inhibitor Cocktail contains individual components including AEBSF 1 10-Phenanthroline Bestatin E-64 Leupeptin and Pepstatin A providing broad specificity against cysteine serine aspartic metalloproteases and aminopeptidases This protease inhibitor cocktail has been optimized and tested specifically for plant cell and tissue extracts Provided as a ready-to-use solution this protease inhibitor cocktail is supplied in DMSO as the solvent
Apexbio Technology LLC Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) 50x(1ml A 1ml B)
Under stable environmental conditions endogenous protein synthesis and degradation are balanced thus maintaining stable cellular protein levels Cell crude extracts contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins in the extracts During in vitro protein extraction from cells and tissues protein synthesis practically ceases while protein degradation increases The optimal way to enhance yields of intact proteins is to add inhibitors for these known enzymes The Protease Inhibitor Cocktail for bacterial cell extracts (100X in DMSO) is designed for bacterial extracts to enhance the stability of proteins This cocktail inhibits proteases responsible for degrading non-phosphorylated or phosphorylated protein substrates This Protease Inhibitor Cocktail is provided as a ready-to-use solution and it is formulated in DMSO
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Seracare Life Sciences Inc 10X PHOSPHATE BUFFERED SALINE
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled.
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS - 20L-CBY
Dulbecco s Phosphate Buffered Saline DPBS - 20L-CBY (For Research Use and Further Manufacturing Only)
Teknova 10X PBS Solution, 4L, Non-Sterile. 4 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.