Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TTTRIS-TRICINE) BUFFER 1
TT (TRIS-TRICINE) BUFFER, 10X SOLUTION: Tris-Tricine Tris-Tricine (TT) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins. TT Buffer offers several advantages over more conventionally used protei...
![eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000165855.jpg-250.jpg)
eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
Chemscene ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.8 (0.2 micron filtered), 500 ml.
eMolecules 2044704-80-3 | tert-butyl 2-(hydroxymethyl)-4-methyl-1H-indole-1-carboxylate | MFCD30489087 | 1g
Ambeed | 2-Bromo-4-(bromomethyl)-1-(trifluoromethyl)benzene | 250mg | 600846886 | A744018 | 372120-77-9 | MFCD13185413 | 317.931 | C8H5Br2F3
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Teknova DNA Suspension Buffer, pH 8.0, 10L, DNase/RNase Tested, PCR Grade, Sterile. 2 Pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Medchemexpress LLC Nvp-tae 226 | 761437-28-9 | MFCD12031516 | 99.8% | 468.94 g·mol⁻¹ | C23H25ClN6O3 | 5 MG
NVP-TAE 226 is an ATP-competitive small-molecule inhibitor of focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), used in cellular and preclinical cancer research to probe signaling pathways and evaluate effects on proliferation, migration, and survival.
- Potent inhibition of FAK and Pyk2 with low-nanomolar IC50s.
- Also inhibits IGF-1R and insulin receptor, enabling study of connected signaling networks.
- Suitable for in vitro assays and preclinical efficacy models.
- Available as solid and solution formulations for flexible dosing.
- High purity appropriate for research applications.
- Stable under recommended storage conditions for prolonged shelf life.
Kd Medical Inc TAE (TRIS-ACETATE EDTA), 50X
TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 8 Bis-Tris Precast
mPAGE 8% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Crescent Chemical Co Inc SERVA TRIS-GLYCINE/SDS BUFFER
SERVA Tris-Glycine/SDS sample buffer (2x) Sample buffer for SDS PAGE. Supplied as 2 x concentrate. Contains 126 mM Tris/HCl (pH 6.8), 20 % glycerol, 4 % SDS and 0.02 % bromophenol blue. Size - 20ML Storage Conditions - +2 °C TO +8 °C Catalog Number - 42527.01
Alkali Scientific Tris Acetate Ultra Pure, 5 Kilograms
Tris Acetate Ultra Pure is a highly pure buffering agent used for creating stable pH conditions in laboratory applications. This 5-kilogram package of tris(acetate) is particularly useful in electrophoresis applications such as DNA and RNA gel electrophoresis. Its ultra-pure grade ensures that it will not interfere with sensitive experiments, making it ideal for molecular biology procedures requiring precise pH control. The tris acetate buffer is widely used in buffer systems for gel electrophoresis, enzymatic reactions, and other applications where stable buffering is critical for optimal results. Its high purity makes it ideal for research applications that demand reliability and accuracy.
Sigma Aldrich Fine Chemicals Biosciences BES >=99.0% (titration) | 10191-18-1 | MFCD00007533 | 25G
BES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 213.25 | 10191-18-1 | MFCD00007533 | 25G
Sigma Aldrich Fine Chemicals Biosciences MES hydrate BioUltra | 1266615-59-1 | MFCD00149409 | 250g
MES hydrate BioUltra | 1266615-59-1 | MFCD00149409 | 250g