Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-500.02.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 25G
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 25G
![Research Products International Corp POPSO, Free Acid, [Piperazine-N-N'-bis(hydroxypropane sulfonic acid)], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P30400-25.0.JPG-250.jpg)
Research Products International Corp POPSO, Free Acid, [Piperazine-N-N'-bis(hydroxypropane sulfonic acid)], 25 Grams
CAS Number: 68189-43-5
Molecular Formula: C10H22N2O8S2
Molecular Weight: 362.4
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-100.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 100 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Research Products International Corp Bicine Ultra-Pure, 500 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 1KG
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 1KG
Kd Medical Inc TAE 10X 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 10 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-10000.0.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 10 Kilograms
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 4 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
Teknova 1X Dulbeccos Phosphate Buffered Saline with 0.5% BSA. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
