Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC PBS Buffer (10x) | 1 L
Phosphate-Buffered Saline (PBS) is a balanced salt solution primarily utilized for various cell culture applications, including washing cells, diluting cells, and preparing reagents. MedChemExpress's PBS Buffer (10x) is filtered, free of Mg2+, Ca2+, and phenol red, and has a pH ranging from 7.2 to 7.4.
- Filtered
- Free of Mg2+
- Free of Ca2+
- Free of phenol red
- pH is 7.2-7.4
General Laboratory Products 2mL 0.85 per. Saline, 100/cs
2mL 0.85 per. Saline with Shelf Life of 1 yr.
Growcells PBS 1X 1L CS12
Ready to use, sterile 1X Phosphate-Buffered Saline solution prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 8g, KCl 0.2g, Na2HPO4 1.44g, KH2PO4 0.24g
Aniara Diagnostica LLC PBS PDR PH 7.4 10L
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed powder mix in sealed pouch yields 10 L 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Used as biological buffer, isotonic. 1 pouch.
AdipoGen PBS Endotoxin-free (sterile), 10 mL
Synonyms: Phosphate Buffer Saline Endotoxin-free (sterile). Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
Rockland Immunochemicals Normal Saline - MB-049-1000
This solution is commonly referred to as physiological saline or isotonic saline.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Crude cell extracts contain a variety of endogenous enzymes capable of degrading proteins such as phosphatases and proteases The optimal method to enhance the yield of intact proteins is to supplement with inhibitors targeting these known enzymes This Protease Inhibitor Cocktail is used in plant cell and tissue extracts to improve protein stability The cocktail inhibits proteases that degrade both non-phosphorylated and phosphorylated protein substrates The Protease Inhibitor Cocktail contains individual components including AEBSF 1 10-Phenanthroline Bestatin E-64 Leupeptin and Pepstatin A providing broad specificity against cysteine serine aspartic metalloproteases and aminopeptidases This protease inhibitor cocktail has been optimized and tested specifically for plant cell and tissue extracts Provided as a ready-to-use solution this protease inhibitor cocktail is supplied in DMSO as the solvent
Apexbio Technology LLC Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) 50x(1ml A 1ml B)
Under stable environmental conditions endogenous protein synthesis and degradation are balanced thus maintaining stable cellular protein levels Cell crude extracts contain numerous endogenous enzymes such as phosphatases and proteases that can degrade proteins in the extracts During in vitro protein extraction from cells and tissues protein synthesis practically ceases while protein degradation increases The optimal way to enhance yields of intact proteins is to add inhibitors for these known enzymes The Protease Inhibitor Cocktail for bacterial cell extracts (100X in DMSO) is designed for bacterial extracts to enhance the stability of proteins This cocktail inhibits proteases responsible for degrading non-phosphorylated or phosphorylated protein substrates This Protease Inhibitor Cocktail is provided as a ready-to-use solution and it is formulated in DMSO
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Apexbio Technology LLC Annexin V-APC/PI Apoptosis Kit 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (APC) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes Additionally this kit can be paired with the nuclear dye propidium iodide (PI) PI specifically binds to double-stranded DNA emitting red fluorescence upon binding but it cannot normally permeate intact cell membranes However PI can penetrate compromised cellular membranes in late-stage apoptotic and dead cells staining nuclei Therefore combined staining with Annexin V-APC and PI allows
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Buffer
DULBECCOS Dulbeccos Phosphate Buffer
Seracare Life Sciences Inc 10X PHOSPHATE BUFFERED SALINE
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled.
Apexbio Technology LLC Annexin V-PE Apoptosis Kit 20tests
Annexin V-PE Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Alternatively you can directly use our ready-to-use dual-staining kits Annexin V-PE/7-AAD Apoptosis Kit ( K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)