Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 9 0) - 2x1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 1kg
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 1kg
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 500G
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 500G
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 50G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 50G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic Acid | 60-00-4 | MFCD00003541 | 100g
Ethylenediaminetetraacetic Acid | Purity: 98% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 100g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid purified grade | 60-00-4 | MFCD00003541 | 1kg
Ethylenediaminetetraacetic acid purified grade | Purity: 98.5% | MW: 292.24 | 60-00-4 | MFCD00003541 | 1kg
Sigma Aldrich Fine Chemicals Biosciences Edetic Acid | 60-00-4 | MFCD00003541 | 200mg
Edetic Acid | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 200mg
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 5mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Kd Medical Inc PBS 10X, PH 7.4, 500ML
10X PBS is concentrated is and ready to be diluted to a 1X working solution. Used as a wash buffer in several research applications. One 500ml bottle.
Kd Medical Inc PBS, 10X, PH 7.4, 4 LITER
10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 4 L cubitainer.
Kd Medical Inc MOPS BUFFER 10X, 1L
10 x MOPS Buffer is intended for use at 1x as the electrophoresis and gel running buffer during the separation of RNA on denaturing formaldehyde/agarose gels. One 1 liter bottle.