Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Abcam 10X ANNEXIN V/ANXA5 BINDING BU ..
10X ANNEXIN V/ANXA5 BINDING BU ..
The product is subject to the following: Abcam Restricted Use Statement
LAB BIOREAGENTS LowBinding VI Buffer 250ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
TARGETMOL CHEMICALS INC NVP-TAE 684 10MG
Also available in 2 mg 5 mg 25 mg 50 mg 100 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. NVP-TAE 684 (TAE684) is a excellently effective and specific ALK inhibitor(IC50=3 nM). purity: 99%
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 5kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a highly purified buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 4 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
BLD PHARMATECH CO LIMITED 4-(Hydroxymethyl)-4-methyltetrahydropyran, CAS Number: 502609-47-4, 1g, Purity: 97%
4-(Hydroxymethyl)-4-methyltetrahydropyran, CAS Number: 502609-47-4, 1g, Purity: 97%
Electron Microscopy Sciences Tris HCL Buffer, pH range 7.2-9.0 250 ML
Please specify pH when ordering.
pH: 7.2-9.0
Bioworld Tricine-SDS Sample Buffer, Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, 0.01% phenol red, and 4% β-mercaptoethanol.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Teknova 5M Sodium Chloride Solution, 200L Bag
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Alkali Scientific Saline 0.85% 20mL, 10/Pk
Saline 0.85% is a sterile isotonic sodium chloride solution widely used in microbiology and laboratory testing. The concentration closely matches the osmotic pressure of bacterial and mammalian cells, helping maintain cellular integrity during handling and dilution. Unlike distilled water, which can cause cells to burst or shrink, this balanced saline preserves cell viability and structure. Because the solution contains no nutrients or buffering components, it provides an inert environment that allows microorganisms to remain stable without stimulating growth. The 20mL tube format offers a convenient volume for washing microbial cells, preparing suspensions, or diluting cultures prior to analysis in microbiological testing and quality control procedures.
Quality Biological Inc TE, pH 7.4 100mL
TE is composed of buffering agent Tris, and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation.
Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc Tris (1M), pH 9.8 1000 mL
Suitable for molecular biology protein chemistry and biochemical applications.
DNase RNase and Protease tested.
Filtered through a 0.22 µm membrane
Applications: Tris Buffer (1M), pH 9.8 is commonly used in SDS-PAGE and Electrophoresis applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.