Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boca Scientific Inc Antibody Stabilizer PBS Base, 50ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
Sigma Aldrich Fine Chemicals Biosciences MOBS >=99% (titration) | 115724-21-5 | MFCD00674916 | 100G
MOBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 223.29 | 115724-21-5 | MFCD00674916 | 100G
Electron Microscopy Sciences Tris Buffer 0.2M, pH 8.0 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 8.0
Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 1KG
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 1KG
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt | 71119-22-7 | MFCD00064350 | 100 g
MOPS sodium salt | Purity: ≥99.5% | Mol Wt: 231.25 | 71119-22-7 | MFCD00064350 | 100 g
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt | 76836-02-7 | MFCD00064349 | 100 g
PIPES disodium salt | Purity: ≥99% | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 100 g
Medchemexpress LLC PIPES (1,4-Piperazinediethanesulfonic acid) | 5625-37-6 | ≥99.0% | 5 G
PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry. It is for research use only.
- Important component of buffer agent
- Prevents glutaraldehyde fixation-induced lipid loss and artifacts when used in buffer
Medchemexpress LLC MES potassium | 39946-25-3 | 99.88% | 100 G
MES (2-Morpholinoethanesulphonic acid) potassium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer and is widely used in biochemistry and molecular biology experiments such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments (e.g., cell culture, enzyme activity determination, electrophoresis, protein studies)
- Zwitterionic buffer, provides effective buffering capacity around pH 6.15
- Pka value changes with temperature, requiring consideration for different temperatures
- Exhibits good buffering properties in biological experiments
- Shows poor affinity with metal ions (e.g., Mg2+, Ca2+, Mn2+), suitable for studies involving metal ions
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 100 g
Tricine | Purity: ≥99% | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 100 g
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 25g
Tricine | Purity: ≥99% (titration) | MW:179.17 | 5704-04-1 | MFCD00004277 | 25g
Kd Medical Inc TRIS HCL, 1M PH 6.8, 500ML
Tris, Hydrochloride is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. 1M solution, pH 6.8. One 500ml bottle.