Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Rockland Immunochemicals 1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED - MB-058
1X TES pH 7.8 DEPC TREATED - TES buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. TES buffer is useful to stabilize proteins, DNA, or RNA, while protecting it from degradation.
Rockland Immunochemicals 1.0 M Tris HCl pH 9.0 - MB-028-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 1KG
Research Products International Corp PIPES FREE ACID ULTRAPURE, 500GM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-1000.03615.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 1 Kilogram
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.
![Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75050-100.0.JPG-250.jpg)
Research Products International Corp HEPES, Sodium Salt [N-(2-Hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)], 100 Grams
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 100G
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 100G
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
Phosphate buffered saline (PBS) is an isotonic buffer SOLUTIONon. This water-based salt SOLUTIONon is made up of disodium hydrogen phosphate sodium chloride and in some preparations potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-1000.0.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 1 Kilogram
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
![Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60050-250.0.JPG-250.jpg)
Research Products International Corp TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 250 Grams
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
LAB BIOREAGENTS NGB I Buffer 250ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.