Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Aniara Diagnostica LLC TBS TBLT PH 7.6 500ML 100 EA
Medicago Tris Buffered Saline 1x (TBS) pH 7.6 supplied as preweighed tablets yields 500 mL 0.050 Tris-HCl, 0.15 M KCl with pH 7.6 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt | 76836-02-7 | MFCD00064349 | 100 g
PIPES disodium salt | Purity: ≥99% | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 100 g
Bioworld TAE Buffer, Quick-Mix Pack (10X), 1 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 10X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, 500 mL
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc HEPES Buffer (1 M), pH 7.3, PK 4 X 100 mL
HEPES is a zwitterionic organic chemical buffering agent ¿ Categorized as an all-purpose buffer ¿ pH 7.2 ¿ 7.6 ¿ Applications: HEPES 1M is used as a buffer alternative from sodium bicarbonate for cell culture. ¿ HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. ¿ HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Performance Tested ¿ pH: 7.2-7.4 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc PBS (10X), pH 7.4, 1000 mL
Balanced salt solution ¿ Wash cells before dissociation ¿ Transport cells or tissue ¿ Dilute cells for counting ¿ Prepare reagents ¿ Endotoxin and Mycoplasma tested ¿ Useful in immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 300 ¿ 55 mOsm/Kg H20 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 Max Shelf Life: 24 months
Quality Biological Inc TAE Buffer 50X, 1000 mL
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc TAE Buffer 10X, 1000 mL
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE is often prepared in concentrated stock solutions of 10X or 50X in the laboratory. ¿ A 1X working solution is prepared prior to electrophoresis. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 41105323 ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 1KG
MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 1KG
Alkali Scientific CellPro™ PBS 10X, pH 7.2, without calcium, magnesium, 500mL, 500ml
CellPro™ PBS, 10X, pH7.2, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
![Aat Bioquest HHBS [Hanks Buffer with 20 mM Hepes] (100 mL)](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502249795.JPG-250.jpg)
Aat Bioquest HHBS [Hanks Buffer with 20 mM Hepes] (100 mL)
Hanks Balanced Salt Solution with 20 mM Hepes buffer HHBS is used for a variety of cell culture applications such as washing cells transporting cells or tissue samples diluting cells for counting and preparing reagents. This catalog is for 100 mL.