Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Rockland Immunochemicals 1.0 M Tris HCl pH 8.5 - MB-027-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of additional buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.
Rockland Immunochemicals 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) - MB-011
Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution). PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration.
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 5mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Cell Signaling Technology Tris-Glycine Transfer Buffer (10X) 1 liter
Tris-Glycine Transfer Buffer (10X) 1 liter
![Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B78000-1000.0.JPG-250.jpg)
Research Products International Corp BIS-TRIS Propane [1,3-Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kilogram
- Substance Name: 1,3-Bis (TRIS(hydroxymethyl)methylamino)propane
- CAS Number: 64431-96-5
- Molecular Formula: C11H26N2O6
- Molecular Weight: 282.3
Research Products International Corp TRIS-Glycine-SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
Composition:
TRIS (Hydroxymethyl) Aminomethane: < 20.0%
SDS: < 6.0%
Research Products International Corp TRIS, ACS Grade, 1 Kilogram
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer.
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-25.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.
Research Products International Corp TRIS BASE, 40% SOLUTION, 4 LITER
A 40% weight by volume solution of TRIS (3.3M TRIS Base) prepared in Molecular Grade Water.
ILEX LIFE SCIENCES LLC PAN-Biotech Dulbecco's Phosphate-Buffered Saline (DPBS), w: Ca and Mg (500 ml)
DPBS, WITH: Ca and Mg. QUANTITY: 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: Dulbecco's Phosphate-Buffered Saline (DPBS) with calcium chloride and magnesium chloride. The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions. These solutions are usually used for washing tissues and cells. Often they are combined with other agents in cell and tissue treatment.
Alkali Scientific CellPro™ Tris HCL 1M pH 8.5, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ Tris HCL, 1M pH 6.8, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ TAE 50X (Tris-Acetate EDTA), 1L
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Alkali Scientific CellPro™ PBS 20X Solution, pH 7.4, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL
CellPro™ 1M HEPES pH 7.5 (Adjusted with KOH) 1000mL