Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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286 – 300 of 3,516 results

286

MP Biomedicals™ PBS 10X

PBS 10X, Rnase, Dnase, Protease free, Filtered through 0,2μm PTFE.

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287

MOBS, 0.2M buffer soln., pH 8.5, Thermo Scientific™

Pricing & Availability
Specifications

CAS	115724-21-5
Molecular Weight (g/mol)	223.29
MDL Number	MFCD00674916
Color	Colorless
Physical Form	Liquid
pH	8.5
SMILES	OS(=O)(=O)CCCCN1CCOCC1
Synonym	4-(4-Morpholinyl)butanesulfonic acid
Recommended Storage	Keep cold
Concentration	0.2 M
IUPAC Name	4-(morpholin-4-yl)butane-1-sulfonic acid
InChI Key	VTOWJTPBPWTSMK-UHFFFAOYSA-N
Molecular Formula	C8H17NO4S

288

AMP, 0.2M buffer soln., pH 9.5, Thermo Scientific™

Pricing & Availability
Specifications

Molecular Weight (g/mol)	89.14
Color	Colorless
Physical Form	Liquid
SMILES	CC(C)(N)CO
Concentration	0.2 M
InChI Key	CBTVGIZVANVGBH-UHFFFAOYSA-N
CAS	124-68-5
MDL Number	MFCD00008051
pH	9.5
Synonym	2-Amino-2-methyl-1-propanol
Recommended Storage	Keep cold
IUPAC Name	2-amino-2-methylpropan-1-ol
Molecular Formula	C4H11NO
Odor	Odorless

289

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Tris, PlusOne™, Cytiva

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)aminomethane PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris(hydroxymethyl)aminomethane
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

290

HEPPS, MP Biomedicals™

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291

Thermo Scientific Chemicals HEPES-buffered saline (2X, pH 7.0), low endotoxin

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Specifications

Name Note	low endotoxin
Storage Note 1	2° to 8°C
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	HEPES-buffered saline (2X, pH 7.0)
TSCA	Yes
Recommended Storage	Keep cold

292

Thermo Scientific Chemicals AMPSO, 98+%

CAS: 68399-79-1 Molecular Formula: C7H17NO5S Molecular Weight (g/mol): 227.28 MDL Number: MFCD00041777 InChI Key: ACERFIHBIWMFOR-UHFFFAOYNA-N Synonym: N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid PubChem CID: 100211 IUPAC Name: 2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid SMILES: CC(C)(CO)NCC(O)CS(O)(=O)=O

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Specifications

PubChem CID	100211
CAS	68399-79-1
Molecular Weight (g/mol)	227.28
MDL Number	MFCD00041777
SMILES	CC(C)(CO)NCC(O)CS(O)(=O)=O
Synonym	N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid
IUPAC Name	2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonic acid
InChI Key	ACERFIHBIWMFOR-UHFFFAOYNA-N
Molecular Formula	C7H17NO5S

293

Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6

For preparation of protein samples for native gel electrophoresis

Pricing & Availability
Specifications

Color	Blue
Physical Form	Liquid
pH	8.6
Chemical Name or Material	Sample Loading Buffer
Concentration	4X
For Use With (Application)	Preparation of protein samples for native gel electrophoresis

294

TRIS-buffered saline (10X, pH 7.4), low endotoxin, Thermo Scientific™

Pricing & Availability
Specifications

Name Note	low endotoxin
MDL Number	MFCD00132476
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	TRIS-buffered saline (10X, pH 7.4)
TSCA	Yes
Recommended Storage	Ambient temperatures

295

Glycylglycine, 0.2M buffer soln., pH 8.0, Thermo Scientific™

CAS: 556-50-3 Molecular Formula: C4H8N2O3 Molecular Weight (g/mol): 132.119 MDL Number: MFCD00008130 InChI Key: YMAWOPBAYDPSLA-UHFFFAOYSA-N PubChem CID: 11163 ChEBI: CHEBI:17201 IUPAC Name: 2-[(2-aminoacetyl)amino]acetic acid SMILES: C(C(=O)NCC(=O)O)N

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Specifications

PubChem CID	11163
CAS	556-50-3
Molecular Weight (g/mol)	132.119
ChEBI	CHEBI:17201
MDL Number	MFCD00008130
SMILES	C(C(=O)NCC(=O)O)N
IUPAC Name	2-[(2-aminoacetyl)amino]acetic acid
InChI Key	YMAWOPBAYDPSLA-UHFFFAOYSA-N
Molecular Formula	C4H8N2O3

296

Physical Form	Liquid
pH	6.5
Chemical Name or Material	MNE buffer
TSCA	Yes
Recommended Storage	Ambient temperatures

297

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PIPES, disodium salt, 99%

CAS: 76836-02-7 Molecular Formula: C8H16N2Na2O6S2 Molecular Weight (g/mol): 346.324 InChI Key: GMHSTJRPSVFLMT-UHFFFAOYSA-L Synonym: 1, 4-Piperazinediethanesulfonic acid disodium salt PubChem CID: 173553 ChEBI: CHEBI:63055 IUPAC Name: disodium;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+]

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Specifications

PubChem CID	173553
CAS	76836-02-7
Molecular Weight (g/mol)	346.324
ChEBI	CHEBI:63055
SMILES	C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+]
Synonym	1, 4-Piperazinediethanesulfonic acid disodium salt
IUPAC Name	disodium;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate
InChI Key	GMHSTJRPSVFLMT-UHFFFAOYSA-L
Molecular Formula	C8H16N2Na2O6S2

298

MilliporeSigma™ MOPS, Sodium, ULTROL™ Grade, Calbiochem™,

CAS: 71119-22-7 Molecular Formula: C7H14NNaO4S Molecular Weight (g/mol): 231.242 InChI Key: MWEMXEWFLIDTSJ-UHFFFAOYSA-M Synonym: 3-(N-Morpholino)propanesulfonic Acid, Na PubChem CID: 3859613 IUPAC Name: sodium;3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CCCS(=O)(=O)[O-].[Na+]

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Specifications

PubChem CID	3859613
CAS	71119-22-7
Molecular Weight (g/mol)	231.242
SMILES	C1COCCN1CCCS(=O)(=O)[O-].[Na+]
Synonym	3-(N-Morpholino)propanesulfonic Acid, Na
IUPAC Name	sodium;3-morpholin-4-ylpropane-1-sulfonate
InChI Key	MWEMXEWFLIDTSJ-UHFFFAOYSA-M
Molecular Formula	C7H14NNaO4S

299

MilliporeSigma™ TAPS, ULTROL™ Grade, Calbiochem™,

CAS: 29915-38-6 Molecular Formula: C7H17NO6S Molecular Weight (g/mol): 243.27 MDL Number: MFCD00007538 InChI Key: YNLCVAQJIKOXER-UHFFFAOYSA-N Synonym: 3-{[tris(Hydroxymethyl)methyl]amino}propanesulfonic Acid PubChem CID: 121591 IUPAC Name: 3-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}propane-1-sulfonic acid SMILES: OCC(CO)(CO)NCCCS(O)(=O)=O

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Specifications

PubChem CID	121591
CAS	29915-38-6
Molecular Weight (g/mol)	243.27
MDL Number	MFCD00007538
SMILES	OCC(CO)(CO)NCCCS(O)(=O)=O
Synonym	3-{[tris(Hydroxymethyl)methyl]amino}propanesulfonic Acid
IUPAC Name	3-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}propane-1-sulfonic acid
InChI Key	YNLCVAQJIKOXER-UHFFFAOYSA-N
Molecular Formula	C7H17NO6S

300

N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, free acid, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

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Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

Life Science Buffers

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