Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Gold Biotechnology Inc HEPES Sodium Salt 1 kg
HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups such as Tris This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers This buffer is capable of forming radicals and is therefore not suitable for redox reactions It is suitable for use in the bicinchoninic acid (BCA) assay
Bio Rad Laboratories XT MOPS RUNNING BUFFER 500ML
Extended range protein standard, 500ul, 50 applications. Product contains 12 bands from 2kD to 250kD. 250, 150, 100, 50, 37, 20, 15, 10 & 5 kD are blue, 75, 25 and 2kD are pink.
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra, for molecular biology, >=99.5% (T) | 5625-37-6 | MFCD00006159 | 50G
PIPES BioXtra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 302.37 | 5625-37-6 | MFCD00006159 | 50G
Sigma Aldrich Fine Chemicals Biosciences HEPES buffer solution 1 M in H2O | 7365-45-9 | MFCD00006158 | 100ml
HEPES buffer solution 1 M in H2O | Mol Wt: | 7365-45-9 | MFCD00006158 | 100ml
Gold Biotechnology Inc Tris HCl 1 kg
Short for Tris (hydroxymethyl) aminomethane (THAM) hydrochloride, Tris HCl is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Gold Biotechnology Inc MOPS, Sodium Salt 1 kg
MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.
Gold Biotechnology Inc Tris (Tris Base) 500 g
GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 250g
BICINE | Purity: 99% | Mol Wt: 163.17 | 150-25-4 | MFCD00004295 | 250g
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3894476 TRIS USP MULTICOM 50KG FLOWMOR
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Zymo Research Corporation DNA Elution Buffer, 50ml
This can be used with any of the DNA purification for DNA elution from columns, plates, and magbeads.
TARGETMOL CHEMICALS INC NVP-TAE 684 10MG
Also available in 2 mg 5 mg 25 mg 50 mg 100 mg 1 mL 10 mM (in DMSO) and bulk. Please contact Fisher for quotes. NVP-TAE 684 (TAE684) is a excellently effective and specific ALK inhibitor(IC50=3 nM). purity: 99%