Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
HEPES Sodium Salt, Biological Buffer, High Purity, 98%, Spectrum™ Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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CAS: 75277-39-3 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.28 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M IUPAC Name: sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1
| CAS | 75277-39-3 |
|---|---|
| Molecular Weight (g/mol) | 260.28 |
| SMILES | [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1 |
| IUPAC Name | sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonate |
| InChI Key | RDZTWEVXRGYCFV-UHFFFAOYSA-M |
| Molecular Formula | C8H17N2NaO4S |
POPSO, 0.2M buffer soln., pH 8.0, Thermo Scientific™
CAS: 108321-07-9 Molecular Formula: C10H20N2Na2O8S2 Molecular Weight (g/mol): 406.376 MDL Number: MFCD00070005 InChI Key: XMRCLLXGMGEVHS-UHFFFAOYSA-L PubChem CID: 53393457 IUPAC Name: disodium;2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazin-1-yl]propane-1-sulfonate SMILES: C1CN(CCN1CC(CS(=O)(=O)[O-])O)CC(CS(=O)(=O)[O-])O.[Na+].[Na+]
| PubChem CID | 53393457 |
|---|---|
| CAS | 108321-07-9 |
| Molecular Weight (g/mol) | 406.376 |
| MDL Number | MFCD00070005 |
| SMILES | C1CN(CCN1CC(CS(=O)(=O)[O-])O)CC(CS(=O)(=O)[O-])O.[Na+].[Na+] |
| IUPAC Name | disodium;2-hydroxy-3-[4-(2-hydroxy-3-sulfonatopropyl)piperazin-1-yl]propane-1-sulfonate |
| InChI Key | XMRCLLXGMGEVHS-UHFFFAOYSA-L |
| Molecular Formula | C10H20N2Na2O8S2 |
Thermo Scientific Chemicals PIPES, 0.5M buffer soln., pH 8.0
CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
| PubChem CID | 79723 |
|---|---|
| CAS | 5625-37-6 |
| Molecular Weight (g/mol) | 302.36 |
| ChEBI | CHEBI:44933 |
| MDL Number | MFCD00006159 |
| SMILES | C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O |
| IUPAC Name | 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | IHPYMWDTONKSCO-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O6S2 |
MOPS, 0.2M buffer soln., pH 7.4, low endotoxin, Thermo Scientific™
CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 4-(3-sulfonatopropyl)morpholin-4-ium SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1
| PubChem CID | 70807 |
|---|---|
| CAS | 1132-61-2 |
| Molecular Weight (g/mol) | 209.26 |
| ChEBI | CHEBI:44115 |
| MDL Number | MFCD00006183 |
| SMILES | [O-]S(=O)(=O)CCC[NH+]1CCOCC1 |
| IUPAC Name | 4-(3-sulfonatopropyl)morpholin-4-ium |
| InChI Key | DVLFYONBTKHTER-UHFFFAOYSA-N |
| Molecular Formula | C7H15NO4S |
Thermo Scientific Chemicals MES, 1.0M buffer soln., pH 7.5
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 |
| MDL Number | MFCD00149409 |
| SMILES | O.OS(=O)(=O)CCN1CCOCC1 |
| IUPAC Name | 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Phosphate Buffered Saline, 0.01 M, pH 7.40 at 25°C, Ricca Chemical
Phosphate Buffered Saline, 0.01 M, pH 7.40 at 25°C is non-Sterile.
StatLab Poly Scientific Phosphate Buffered Saline 0.1M pH 7.2
For preserving tissues during surgical procedures and IHC staining procedures.
Tris(hydroxymethyl)aminomethane 99.0+%, TCI America™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trimethylolaminomethane, Tris-Base PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trimethylolaminomethane, Tris-Base |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Buffer Solution A, for Sulfate Analysis (Turbidimetric Method), Ricca Chemical
For Analysis of Sulfate (Turbidimetric Method)
| Color | Colorless |
|---|---|
| Packaging | Natural Poly Bottle |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution |
| Grade | Chemical Solution |
| For Use With (Application) | Sulfate Analysis (Turbidimetric Method) |
| Solution Type | Buffer |
| Melting Point | ∼ 0°C |
StatLab Phosphate Buffered Saline, pH 7.2
Buffers are made from the highest quality chemicals using a specialized process which ensures exact pH levels.
| Grade | Reagent |
|---|
Buffer Solution, for Nitrate Determination using Ion Selective Electrode, Ricca Chemical
Nitrate Determination using Ion Selective Electrode
| Color | Colorless |
|---|---|
| Packaging | Amber Glass Bottle |
| Physical Form | Liquid |
| pH | 2.98 to 3.98 |
| Chemical Name or Material | Buffer Solution |
| Grade | Chemical Solution |
| For Use With (Application) | Ion Selective Analysis |
| Solution Type | Buffer |
| Melting Point | ∼ 0°C |
N,N-Di(2-hydroxyethyl)glycine 99.0+%, TCI America™
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| Synonym | bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine |
| IUPAC Name | sodium 2-[bis(2-hydroxyethyl)amino]acetate |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
| Percent Purity | 99.8% to 100.2% |
|---|---|
| CAS | 77-86-1 |
| For Use With (Equipment) | Instruments and Electrodes |
| Molecular Weight (g/mol) | 121.135 |
| Physical Form | Crystalline Powder |
| pH | 10.0 to 11.5 for 5% solution |
| Grade | ACS |
| For Use With (Application) | Laboratory |
| Solution Type | Standard |