Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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496 – 510 of 3,492 results

496

MilliporeSigma™ TAE Buffer, 10X, Molecular Biology Grade, Calbiochem™,

A 10X concentrate that can be diluted to a 1X solution containing 40mM Tris, 40mM acetate, and 1mM EDTA

Pricing & Availability

497

StatLab Phosphate Buffered Saline, pH 7.2

Buffers are made from the highest quality chemicals using a specialized process which ensures exact pH levels.

Pricing & Availability
Specifications

Grade	Reagent

498

Tris(hydroxymethyl)aminomethane 99.0+%, TCI America™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trimethylolaminomethane, Tris-Base PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	2-Amino-2-(hydroxymethyl)-1,3-propanediol, Trimethylolaminomethane, Tris-Base
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

499

N,N-Di(2-hydroxyethyl)glycine 99.0+%, TCI America™

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine
IUPAC Name	sodium 2-[bis(2-hydroxyethyl)amino]acetate
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

500

Buffer Solution, for Nitrate Determination using Ion Selective Electrode, Ricca Chemical

Nitrate Determination using Ion Selective Electrode

Pricing & Availability
Specifications

Color	Colorless
Packaging	Amber Glass Bottle
Physical Form	Liquid
pH	2.98 to 3.98
Chemical Name or Material	Buffer Solution
Grade	Chemical Solution
For Use With (Application)	Ion Selective Analysis
Solution Type	Buffer
Melting Point	∼ 0°C

501

TRIS Buffer, 1 M, pH 7.50, Ricca Chemical

Ready-to-use 1 Molar TRIS buffer at pH 7.50, designed for general laboratory use.

Pricing & Availability

502

Buffer Solution A, for Sulfate Analysis (Turbidimetric Method), Ricca Chemical

For Analysis of Sulfate (Turbidimetric Method)

Pricing & Availability
Specifications

Color	Colorless
Packaging	Natural Poly Bottle
Physical Form	Liquid
Chemical Name or Material	Buffer Solution
Grade	Chemical Solution
For Use With (Application)	Sulfate Analysis (Turbidimetric Method)
Solution Type	Buffer
Melting Point	∼ 0°C

503

Phosphate Buffered Saline, 0.01 M, pH 7.40 at 25°C, Ricca Chemical

Phosphate Buffered Saline, 0.01 M, pH 7.40 at 25°C is non-Sterile.

Pricing & Availability

504

StatLab MasterTech Phosphate Buffered Saline

A crucial reagent for washing cells and tissues.

Pricing & Availability

505

StatLab Poly Scientific Phosphate Buffered Saline 0.1M pH 7.2

For preserving tissues during surgical procedures and IHC staining procedures.

Pricing & Availability

506

Tris(hydroxymethyl)aminomethane, ACS Reagent (Tris; THAM; 2-Amino-2-(hydroxymethyl)-1,3-propanediol), Reagents

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tromethamine IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: NC(CO)(CO)CO

Pricing & Availability
Specifications

CAS	77-86-1
Molecular Weight (g/mol)	121.14
SMILES	NC(CO)(CO)CO
Synonym	Tromethamine
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

507

Percent Purity	99.8% to 100.2%
CAS	77-86-1
For Use With (Equipment)	Instruments and Electrodes
Molecular Weight (g/mol)	121.135
Physical Form	Crystalline Powder
pH	10.0 to 11.5 for 5% solution
Grade	ACS
For Use With (Application)	Laboratory
Solution Type	Standard