Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
PIPES, disodium salt, 99%
CAS: 76836-02-7 Molecular Formula: C8H16N2Na2O6S2 Molecular Weight (g/mol): 346.324 InChI Key: GMHSTJRPSVFLMT-UHFFFAOYSA-L Synonym: 1, 4-Piperazinediethanesulfonic acid disodium salt PubChem CID: 173553 ChEBI: CHEBI:63055 IUPAC Name: disodium;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+]
| PubChem CID | 173553 |
|---|---|
| CAS | 76836-02-7 |
| Molecular Weight (g/mol) | 346.324 |
| ChEBI | CHEBI:63055 |
| SMILES | C1CN(CCN1CCS(=O)(=O)[O-])CCS(=O)(=O)[O-].[Na+].[Na+] |
| Synonym | 1, 4-Piperazinediethanesulfonic acid disodium salt |
| IUPAC Name | disodium;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate |
| InChI Key | GMHSTJRPSVFLMT-UHFFFAOYSA-L |
| Molecular Formula | C8H16N2Na2O6S2 |
MilliporeSigma™ MOPS, Sodium, ULTROL™ Grade, Calbiochem™,
CAS: 71119-22-7 Molecular Formula: C7H14NNaO4S Molecular Weight (g/mol): 231.242 InChI Key: MWEMXEWFLIDTSJ-UHFFFAOYSA-M Synonym: 3-(N-Morpholino)propanesulfonic Acid, Na PubChem CID: 3859613 IUPAC Name: sodium;3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CCCS(=O)(=O)[O-].[Na+]
| PubChem CID | 3859613 |
|---|---|
| CAS | 71119-22-7 |
| Molecular Weight (g/mol) | 231.242 |
| SMILES | C1COCCN1CCCS(=O)(=O)[O-].[Na+] |
| Synonym | 3-(N-Morpholino)propanesulfonic Acid, Na |
| IUPAC Name | sodium;3-morpholin-4-ylpropane-1-sulfonate |
| InChI Key | MWEMXEWFLIDTSJ-UHFFFAOYSA-M |
| Molecular Formula | C7H14NNaO4S |
Tris Base Buffer, Powder, Corning™
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Used in buffer solutions in biochemical and molecular biological research
| MDL Number | MFCD00236358 |
|---|---|
| Packaging | Plastic bottle |
| Physical Form | Liquid |
| Chemical Name or Material | TBE (5X) |
| TSCA | Yes |
| Recommended Storage | Ambient temperatures |
Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Molecular Biology Grade, 99.9%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | TRIS |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.5, 0.2 micron filtered
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals Potassium phosphate, 0.2M buffer soln., pH 7.4
CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O
| CAS | 7778-77-0 |
|---|---|
| Molecular Weight (g/mol) | 136.08 |
| MDL Number | MFCD00011401 MFCD00147253 |
| SMILES | [K+].OP(O)([O-])=O |
| IUPAC Name | potassium dihydrogen phosphate |
| InChI Key | GNSKLFRGEWLPPA-UHFFFAOYSA-M |
| Molecular Formula | H2KO4P |
N,N-Di(2-hydroxyethyl)glycine 99.0+%, TCI America™
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: sodium 2-[bis(2-hydroxyethyl)amino]acetate SMILES: [Na+].OCCN(CCO)CC([O-])=O
| PubChem CID | 8761 |
|---|---|
| CAS | 150-25-4 |
| Molecular Weight (g/mol) | 185.16 |
| ChEBI | CHEBI:40957 |
| MDL Number | MFCD00004295 |
| SMILES | [Na+].OCCN(CCO)CC([O-])=O |
| Synonym | bicine,n,n-bis 2-hydroxyethyl glycine,diethylolglycine,dihydroxyethylglycine,diethanol glycine,n,n-di 2-hydroxyethyl glycine,bicene,2-bis 2-hydroxyethyl amino acetic acid,n,n-dihydroxyethyl glycine,n,n-dihydroxyethylglycine |
| IUPAC Name | sodium 2-[bis(2-hydroxyethyl)amino]acetate |
| InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
| Molecular Formula | C6H12NNaO4 |
Buffer Solution, for Nitrate Determination using Ion Selective Electrode, Ricca Chemical
Nitrate Determination using Ion Selective Electrode
| Color | Colorless |
|---|---|
| Packaging | Amber Glass Bottle |
| Physical Form | Liquid |
| pH | 2.98 to 3.98 |
| Chemical Name or Material | Buffer Solution |
| Grade | Chemical Solution |
| For Use With (Application) | Ion Selective Analysis |
| Solution Type | Buffer |
| Melting Point | ∼ 0°C |
Buffer Solution A, for Sulfate Analysis (Turbidimetric Method), Ricca Chemical
For Analysis of Sulfate (Turbidimetric Method)
| Color | Colorless |
|---|---|
| Packaging | Natural Poly Bottle |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution |
| Grade | Chemical Solution |
| For Use With (Application) | Sulfate Analysis (Turbidimetric Method) |
| Solution Type | Buffer |
| Melting Point | ∼ 0°C |
StatLab Poly Scientific Phosphate Buffered Saline 0.1M pH 7.2
For preserving tissues during surgical procedures and IHC staining procedures.
Tris(hydroxymethyl)aminomethane, ACS Reagent (Tris; THAM; 2-Amino-2-(hydroxymethyl)-1,3-propanediol), Reagents
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tromethamine IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: NC(CO)(CO)CO
| CAS | 77-86-1 |
|---|---|
| Molecular Weight (g/mol) | 121.14 |
| SMILES | NC(CO)(CO)CO |
| Synonym | Tromethamine |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
| Percent Purity | 99.8% to 100.2% |
|---|---|
| CAS | 77-86-1 |
| For Use With (Equipment) | Instruments and Electrodes |
| Molecular Weight (g/mol) | 121.135 |
| Physical Form | Crystalline Powder |
| pH | 10.0 to 11.5 for 5% solution |
| Grade | ACS |
| For Use With (Application) | Laboratory |
| Solution Type | Standard |