Life Science Buffers
Edvotek™ Electrophoresis Buffers
Buffers for use in the science classroom.
Innovating Science™ Agarose Gel Reagents: TBE Buffer
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris Hydrochloride, Ultrapure, 500 g, Innovating Science™
CAS: 1185-53-1 Molecular Weight (g/mol): 157.6
Tris Base, Ultrapure, 500 g, Innovating Science™
CAS: 77-86-1 Molecular Weight (g/mol): 121.14
Gold Biotechnology Inc MOPS Free Acid, Ultra Pure 1 kg
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.
Cytiva HBS-P+ Buffer 10, 4x50mL
Concentrated stock solution containing 0.1 M HEPES, 1.5 M NaCl and 0.5% v/v Surfactant P20. Will yield pH 7.4 when diluted 10X. Supports the recommendations for small molecule assays in Biacore systems. For all systems.
Cytiva HBS-EP+ Buffer 10, 1000mL
General purpose running buffer. Concentrated stock solution yielding pH 7.4 when diluted 10x. For use in Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100
Cytiva PBS-P+ Buffer 10, 1000mL
Phosphate buffered saline containing detergent, which is recommended as running buffer for small molecule analysis in Biacore systems. Concentrated stock solution, yielding pH 7.4 when diluted 10X and supplemented with 2% DMSO. PBS buffer with DMSO is commonly used for small molecule compound samples.
Cytiva HBS-N Buffer 10, 4x50mL
Contents: 0.1 M HEPES, 1.5 M NaCl; Running buffer. Concentrated stock solution, yielding pH 7.4 when diluted 10X. For use in all Biacore SPR systems.