Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [10X]](https://assets.fishersci.com/TFS-Assets/CCG/G-Biosciences/product-images/PBS_buffer.jpg-250.jpg)
G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [10X]
10X PBS is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer saline.
Innovating Science™ Agarose Gel Reagents: TBE Buffer
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris Base, Ultrapure, 500 g, Innovating Science™
CAS: 77-86-1 Molecular Weight (g/mol): 121.14
Cytiva HBS-P+ Buffer 10, 4x50mL
Concentrated stock solution containing 0.1 M HEPES, 1.5 M NaCl and 0.5% v/v Surfactant P20. Will yield pH 7.4 when diluted 10X. Supports the recommendations for small molecule assays in Biacore systems. For all systems.
Cytiva HBS-EP+ Buffer 10, 1000mL
General purpose running buffer. Concentrated stock solution yielding pH 7.4 when diluted 10x. For use in Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100
Cytiva PBS Buffer 10, 1000mL
Contents: 0.1 M phosphate buffer with 27 mM KCl and 1.37 M NaCl, 1 1000 ml; Running buffer. Concentrated stock solution, yielding pH 7.4 when diluted 10X and supplemented with 5% DMSO. (PBS buffer with DMSO is commonly used for small molecule assays.)
Cytiva HBS-N Buffer 10, 4x50mL
Contents: 0.1 M HEPES, 1.5 M NaCl; Running buffer. Concentrated stock solution, yielding pH 7.4 when diluted 10X. For use in all Biacore SPR systems.
Cytiva HBS-P+ Buffer 10, 1000mL
Concentrated stock solution containing 0.1 M HEPES, 1.5 M NaCl and 0.5% v/v Surfactant P20. Will yield pH 7.4 when diluted 10X. Supports the recommendations for small molecule assays in Biacore systems. For all systems.
Cytiva HBS-N Buffer 10, 1000mL
Contents: 0.1 M HEPES, 1.5 M NaCl; Running buffer. Concentrated stock solution, yielding pH 7.4 when diluted 10X. For use in all Biacore SPR systems.
Cytiva PBS-P+ Buffer 10, 1000mL
Phosphate buffered saline containing detergent, which is recommended as running buffer for small molecule analysis in Biacore systems. Concentrated stock solution, yielding pH 7.4 when diluted 10X and supplemented with 2% DMSO. PBS buffer with DMSO is commonly used for small molecule compound samples.