Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Tris Hydrochloride, Ultrapure, 500 g, Innovating Science™
CAS: 1185-53-1 Molecular Weight (g/mol): 157.6
Innovating Science™ Agarose Gel Reagents: TBE Buffer
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
![G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [1X]](https://assets.fishersci.com/TFS-Assets/CCG/G-Biosciences/product-images/PBS_buffer.jpg-250.jpg)
G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [1X]
1X PBS (Phosphate Buffered Saline) is a ready-to-use, sterile, pH adjusted blend of phosphate buffer saline.
Tris Base, Ultrapure, 500 g, Innovating Science™
CAS: 77-86-1 Molecular Weight (g/mol): 121.14
Dulbecco's Phosphate Buffered Saline 1X, pH 7.4, Bioreagent Grade, Sterile-Filtered, VION™
Provides the precise isotonic environment and physiological pH required to maintain molecular integrity during sensitive laboratory workflows.
G-Biosciences Phosphate Buffered Saline (PBS), Sterile, [10X]
10X PBS is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer saline.
Tris EDTA Buffer 1X, pH 8.3, Bioreagent Grade, Sterile-Filtered, VION™
A stabilized solution for the long-term preservation of genomic and plasmid DNA.
Tris Borate EDTA Buffer 1X, pH 8.3, Bioreagent Grade, Sterile-Filtered, VION™
Engineered for high-voltage stability and superior resolution of small DNA and RNA fragments. Provides the high buffering capacity required for extended electrophoresis runs without the pH drift common in standard reagents.
Tris Acetate EDTA Buffer 1X, pH 8.3, Bioreagent Grade, Sterile-Filtered, VION™
This TAE buffer formulation ensures maximum sample recovery during gel extraction by eliminating impurities that interfere with downstream ligation and cloning.
Phosphate Buffered Saline 1X, pH 7.4, Bioreagent Grade, Sterile-Filtered, VION™
Strictly isotonic and manufactured to be non-toxic, serving as the reliable standard for cell washing, dilutions, and reagent preparation.
Tris Glycine SDS Buffer 1X, Bioreagent Grade, Sterile-Filtered, VION™
The standard buffer for denaturing electrophoresis.
Tris Glycine SDS Buffer 10X, Bioreagent Grade, Sterile-Filtered, VION™
This formulation provides a uniform mass-to-charge ratio for all proteins, delivering highly reproducible molecular weight determinations.
