Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Tris-HCL Buffer 1M, pH 10.2, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
![eMolecules 467454-51-9 | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158706.JPG-250.jpg)
eMolecules 467454-51-9 | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718
tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | 467454-51-9 | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718
Crescent Chemical Co Inc SERVA TRIS-TRICINE/SDS BUFFER
SERVA Tris-Tricine/SDS electrophoresis buffer (10x) Running buffer for SDS PAGE. Supplied as 10 x concentrate. Contains 1 M Tris, 1 M Tricine and 1 % SDS. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42552.01
Bioworld TBE Urea Sample Buffer (2X), 25 mL
Product DescriptionTBE(Tris-Borate-EDTA) urea sample buffer is used for nucleic acid analysis. It has a 2X concentration and contains 45 mM Tris base, 45 mM Boric acid, 6% Ficol, 3.5M Urea, 0.005% Bromophenol blue, 0.025% Xylene and 1 mM EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 500g
BIS-TRIS | Purity: 98% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500g
Chem Service Inc Tris(1,3-dichloroisopropyl)phosphate | 13674-87-8 | MFCD00083121 | 250mg
Tris(1,3-dichloroisopropyl)phosphate | MW: 430.88 | 13674-87-8 | MFCD00083121 | 250mg
Bioworld TAE Buffer, Quick-Mix Pack (25X), 1 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 25X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Growcells TAE BUFFER 10X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 48.46g TRIS Base, 11.4ml Acetic Acid, 3.72g EDTA. pH 8.3
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS 98.0 (titration), 6976-37-0, MFCD00002853
BIS-TRIS 98.0 (titration)
STEMCELL Technologies RoboSep™ Buffer 2, Size: 250 mL
RoboSep™ Buffer 2 is recommended for select EasySep™ cell separation protocols. Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Bioworld TTE Buffer, Ready-Pack (20X), 1 PK(s)
20x TTE (Tris-TAPS-EDTA) buffer pack can be dissolved into 2L of water to make 2L of 1x TTE solution.
Teknova 1M Tris-HCl, pH 7.4. 1000 mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld TBS Buffer, High Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is high salt used for Western blot washing with a concentration of 10X. It contains 0.5M Tris, 4.5M NaCl and has a pH level of 7.4. Buffer is stored at room temperature.TBS is isotonic and non-toxic, and is used to dilute substances. It is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.