Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 50X TAE, Electrophoresis Grade. (2M Tris-Acetate, 100mM EDTA) 1000mL, Non-Sterile. 2 pack.
2M Tris-Acetate, 100mM EDTA
Teknova DNA Suspension Buffer, pH 8.0. DNase/RNase Tested, PCR Grade. 1mL Tubes, 50-Pack, Sterile.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
ILEX LIFE SCIENCES LLC PAN-Biotech Dulbecco's Phosphate-Buffered Saline (DPBS), w: Ca and Mg (500 ml)
DPBS, WITH: Ca and Mg. QUANTITY: 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: Dulbecco's Phosphate-Buffered Saline (DPBS) with calcium chloride and magnesium chloride. The main purpose of buffered salt solutions is the maintenance of pH and osmolarity in cell culture applications as well as providing essential inorganic ions. These solutions are usually used for washing tissues and cells. Often they are combined with other agents in cell and tissue treatment.
Teknova 1X PBS, Dulbecco's Solution (with Calcium and Magnesium). 1000mL, Sterile 2 pack.
137mM Sodium chloride, 0.5mM Magnesium chloride, 8.1mM Disodium phosphate, 0.9mM Calcium chloride, 1.47mM Monopotassium phosphate, 2.7mM Potassium chloride