Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M HEPES, pH 7.5, 20L Bag, Sterile. 4 Pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
ILEX LIFE SCIENCES LLC PAN-Biotech HEPES Buffer 1M (100 ml)
HEPES Buffer 1M. QUANTITY: 100 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. COMPOSITION: HEPES 238.31 g/L.
Medchemexpress LLC MES hydrate | 1266615-59-1 | MFCD00149409 | 99.9% | 10 G
MES (2-Morpholinoethanesulphonic acid) hydrate is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely used in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies. This zwitterionic buffer provides effective buffering capacity around pH 6.15. Its pKa value changes with temperature, a factor to consider when used at varying temperatures. MES hydrate exhibits good buffering properties in biological experiments and has a poor affinity for metal ions, making it suitable for studies involving such ions.
- Amphoteric ion buffer
- Buffer capacity ranging pH 5.5-7.0
- Widely used in biochemistry and molecular biology experiments
- Effective buffering capacity at approximately pH 6.15
- Poor affinity with metal ions
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005335 HEPES EMPROVE EVOLVE 100G
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659782.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 5 Kg
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical buffer available in USP-grade, designed for a wide range of scientific and research applications. This 5kg container is ideal for laboratories that require large volumes of buffer solution for high-throughput processes, including protein studies, enzymatic assays, and cell culture applications. PIPES is known for its ability to maintain a stable pH under various conditions where other buffers may not be suitable. It is especially effective in high-salt and high-temperature environments. The 5kg pack ensures that large-scale laboratory projects can proceed without the need for frequent reordering, making it both a cost-effective and convenient solution.
eMolecules 913642-78-1 | tert-Butyl 2-(2-hydroxyethyl)morpholine-4-carboxylate | ChemScene | MFCD09608014 | 231.292 | C11H21NO4 | 97.000 | CC(C)(C)OC(=O)N1CCOC(CCO)C1 | 100mg | 596346186
tert-Butyl 2-(2-hydroxyethyl)morpholine-4-carboxylate | ChemScene | 913642-78-1 | MFCD09608014 | 231.292 | C11H21NO4 | 97.000 | CC(C)(C)OC(=O)N1CCOC(CCO)C1 | 100mg | 596346186
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from adenosine deaminase, commonly employed in biochemical research, particularly for preparing enzyme buffers.
- Used in biochemical research.
- Specifically used in the preparation of enzyme buffers.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 5 MG
AR-13324 analog mesylate is an analog of AR-13324. This product is for research use only.
- Acts as a Rho-associated protein kinase (ROCK) inhibitor.
- Functions as a norepinephrine transporter (NET) inhibitor.
Bioworld ACES Buffer, 0.5M, pH 7.5, 250 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Alkali Scientific MES, Anhydrous, 25 G
MES, Anhydrous, 25 G, is a small-volume, high-purity buffering agent used to maintain pH stability in laboratory experiments. With its anhydrous form, it dissolves readily in solvents for precise pH adjustments. MES buffers in the pH range of 5.5 to 6.7, making it particularly useful in applications such as electrophoresis, enzyme assays, and in preparing cell culture media. The 25 g package is ideal for small-scale experiments or for laboratories that require frequent pH adjustments but do not need larger quantities of the reagent. MES is an essential tool for many biochemical and biological processes that require exacting conditions for reproducible results.