Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661332.jpg-250.jpg)
Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 100 G
HEPPSO (N-(2 Hydroxyethyl) piperazine N'-(2 hydroxypropanesulfonic acid)) is a biochemical reagent supplied in a 100g bottle. This buffering agent is commonly used in laboratories for maintaining a stable pH, particularly in molecular biology and biochemistry applications. HEPPSO is widely used for enzyme assays, protein purification, and chromatography, as well as in the preparation of biological samples where precise pH control is necessary. This high-quality reagent ensures consistent results in experiments that require a stable and controlled acidic environment, especially in cell culture, electrophoresis, and other biological assays.
![Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661289.jpg-250.jpg)
Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 500 G
TAPS, or Tris(hydroxymethyl) aminopropanesulfonic acid, is available in a high-purity free acid form for laboratory use. The 500g size is ideal for medium-scale buffer preparation, particularly in research and diagnostics. TAPS is widely used in biochemical, molecular biology, and clinical research applications to maintain a stable pH during experiments. The 500g quantity allows for frequent preparation of buffers without the need for constant restocking, providing researchers with a reliable, long-term supply. TAPS is critical for pH-sensitive reactions, including protein assays, electrophoresis, and enzyme studies, ensuring accuracy and consistency in experimental conditions.
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 100G Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.02 CAS 4432-31-9
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.03 CAS 4432-31-9
Bioworld PEB- Protein Extraction Buffer (5x), 500 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
![eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000165855.jpg-250.jpg)
eMolecules 1214727-57-7 | TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
TRANS-TERT-BUTYL (3-(AMINOMETHYL)CYCLOBUTYL)CARBAMATE | AstaTech | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 95.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 449729861
ILEX LIFE SCIENCES LLC TRYPSIN P10-021500 500ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (500 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN P10-021500 6 X 500ML
PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg (6 x 500 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC TRYPSIN/EDTA P10-024100 100ML
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in DPBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.50% Trypsin. EDTA: 0.20% (5.3 mM) EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).