Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc PBS Solution With Sodium Azide, 500 mL, Antimicrobial Preservative
PBS W/SODIUM AZIDE 500ML is a buffered saline solution designed for laboratory applications requiring a preservative to inhibit microbial growth. This product not only provides a stable wash and storage buffer but also enhances the longevity of biological samples. The inclusion of sodium azide ensures effective preservation, differentiating it from standard saline solutions. This versatile product is essential for researchers seeking reliable and long-lasting buffer solutions.
Rockland Immunochemicals 1.0 M HEPES pH 7.2 - MB-062-0100
HEPES is a useful buffer for pH's ranging from 6.8-8.2. This makes HEPES ideal for cell culturing because of its usefulness in maintaining pH changes in presence of carbon dioxide production from cellular respiration.1.0 M HEPES pH 7.2 is ideal for investigators in Molecular Biology, Cell Biology, and Immunology researchers.
Sigma Aldrich Fine Chemicals Biosciences PIPES Dipotassium Salt | 108321-27-3 | MFCD00069751 | 100g
PIPES Dipotassium Salt | Purity: 99% | Mol Wt: 378.55 | 108321-27-3 | MFCD00069751 | 100g
STEMCELL Technologies Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum, Size: 500 mL
Dulbecco's Phosphate Buffered Saline with 2% Fetal Bovine Serum is recommended for use as a buffer to dilute or wash nucleated cells.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Low glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This modification has been shown to improve the stability of the medium.
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Hampton Research 100% TACSIMATE PH 8.0 200ML
HR2-829/100% Tacsimate pH 8.0 - 200 ml. Please note items from this supplier are non-returnable.