Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific Protease Inhibitor Cocktail, EDTA Free, 20 Tablets
Protease Inhibitor Cocktail, EDTA-Free (Tablet Form), is a versatile enzyme inhibitor formulation that effectively prevents protease activity during protein extraction. Offered in a convenient tablet form, this product allows for easy and precise inhibitor preparation in a range of biological samples. The EDTA-free formulation is particularly useful when metal ion-dependent proteases need to be targeted without interfering with sample components. This product is widely used for protein isolation from tissues and cells, particularly in applications like immunoassays, Western blotting, and enzyme activity studies, where protein integrity is critical.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbecco Phosphate Buffered Saline (DPBS) is a balanced salt SOLUTIONon.
Biotang Inc Phosphate-Buffered Saline PBS, 10X, pH 7.6, 1000 ml
Phosphate-Buffered Saline PBS, 10X, pH 7.6, 1000 ml.
ILEX LIFE SCIENCES LLC TRYPSINEDTA P10-024100 6X100ML
PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in DPBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 0.5% / EDTA 0.2% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme plus EDTA in a 10x concentrated PBS solution. Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.50% Trypsin. EDTA: 0.20% (5.3 mM) EDTA. STERILE: Yes. STORAGE TEMP.: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Ethos Biosciences Dilurex PBS Casein Sucrose Blocker, 1000ml, Ethos
Dilurex PBS Casein Sucrose Blocking Buffer offers the best price to performance ratio on the market. The base system is phosphate buffered saline - perfect for systems that are using PBS as a diluent or reaction buffer. Casein is used as the blocking protein which binds to unoccupied hydrophobic or charged sites on assay surfaces preventing unwanted non-specific binding of antibodies, enzymes, or other proteins leading to clearer, more reliable signals. Sucrose stabilizes your mixture by preserving the folding integrity of proteins during drying or freezing. Choose one of our other blocking buffers if your assay is detecting phosphoproteins, uses biotin/streptavidin detection, Alkaline Phosphatase detection, autoimmune assays, or aldehyde coated substrates in case of potential interference.
Alkali Scientific CellPro™ PBS, 10X, pH 7.4, 500mL, 500ml, 10/case
CellPro™ PBS, 10X, pH7.4, 500mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Teknova 20X PBS Solution, pH 7.2 (at 1X). 10L, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
Suitable for growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661398.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are solid tablets designed for easy dissolution in water, making it simple to prepare 200mL of phosphate-buffered saline. These tablets are ideal for laboratories that frequently need to prepare large volumes of PBS for use in cell culture, microbiology, and molecular biology research. The tablets dissolve quickly and efficiently, ensuring that the resulting solution maintains the proper pH and osmolarity for reliable experimental results. Each bottle contains 100 tablets, providing an economical and convenient way to prepare PBS without the need for precise measuring or mixing of individual components.
Apexbio Technology LLC Annexin V-APC Reagent 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) Under normal conditions PS is located on the inner side of the plasma membrane lipid bilayer however during early apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane becoming exposed to the extracellular environment Annexin V has a high affinity for PS Fluorescent (APC)-labeled Annexin V serves as a fluorescent probe enabling detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining procedure takes only 15-30 minutes to complete Use of this reagent requires additional purchase of 10X Binding Buffer (Catalog no K2284) To further characterize different apoptosis stages Annexin V can be combined with nuclear dyes such as 7-AAD DAPI PI or SYTOX Green These nuclear dyes specifically bind double-stranded DNA generating fluorescence Normally they cannot penetrate intac