Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Tris-Glycine-SDS Buffer 101L
Tris-glycine-SDS (TGS) running buffer is the most commonly used buffer for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. TGS is usually used for both anode and cathode buffer. Recommended running conditions is 150 volts for mini vertical gel electrophoresis units.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer 5x1L
TRIS Tris-Borate-EDTA buffer 5x1L
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt solutin500ML
HEPES is a general-purpose zwitterionic buffer which does not bind magnesium calcium manganese(II) or copper(II) ions. HEPES has been classified as Good buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
Sigma Aldrich Fine Chemicals Biosciences HEPES Buffered Saline Solu
HEPES Buffered Saline SOLUTIONon
Quality Biological Inc PBS (10X), pH 7.4, 20 Liters
Balanced salt solution ¿ Wash cells before dissociation ¿ Transport cells or tissue ¿ Dilute cells for counting ¿ Prepare reagents ¿ Endotoxin and Mycoplasma tested ¿ Useful in immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 300 ¿ 55 mOsm/Kg H20 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ Max Shelf Life: 24 months
Quality Biological Inc PBS pH 7.2, 4 Liters
PBS (phosphate buffered saline) is a balanced salt solution ¿ Used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents ¿ Endotoxin and Mycoplasma tested ¿ Also useful in immunological applications, such as Western Blotting ¿ Storage Condition: 15¿C ¿ 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.1 -7.3 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc DPBS without Calcium and Magnesium, 10 Liters
DPBS is a balanced salt solution (BSS) ¿ Used for the handling and culturing of mammalian cells. ¿ Calcium and magnesium facilitate cell binding and clumping. ¿ DPBS without these ions can be used to wash and rinse suspended cells. ¿ Applications: DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate Liters ¿ Wash the wells of microtiter plates ¿ Wash cells during preparation and serial transfer. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
![Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 1702.9 Grams of Powder Makes 10 Liters of Solution](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T22020-10.0.JPG-250.jpg)
Research Products International Corp TBE Buffer [TRIS-Borate-EDTA], 10X Powder, 1702.9 Grams of Powder Makes 10 Liters of Solution
Dry homogeneous blend of molecular biology grade TRIS base, Boric Acid and EDTA.
For use in gel electrophoresis.
Rockland Immunochemicals 1X TES pH 7.8 (10 mM Tris HCl, 100 mM NaCl, 1 mM EDTA) DEPC TREATED - MB-058
1X TES pH 7.8 DEPC TREATED - TES buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. TES buffer is useful to stabilize proteins, DNA, or RNA, while protecting it from degradation.
S2 Media 1L 1x Phosphate Buffered Saline (PBS), pH 6.0, 1L Media Bottle, 1ea
S2Media™ 1x Phosphate Buffered Saline (PBS), pH 6.0 has many applications due to its isotonic nature, including uses as a rinse fluid, diluent, and buffer. Phosphate salts are nontoxic to living cells and have a high buffering capacity. pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. PBS is generally used as a 10mM working solution containing a mixture of sodium chloride and inorganic phosphate salts.• Product meets AOAC, APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• PBS should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.