Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 1M Tris-HCl, pH 8.0 Solution. 4L, Sterile. 4 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 20x PBS with 0.05% Tween-20. 500mL, Sterile. 8 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Medchemexpress LLC PBS Buffer (10x) | 1 L
Phosphate-Buffered Saline (PBS) is a balanced salt solution primarily utilized for various cell culture applications, including washing cells, diluting cells, and preparing reagents. MedChemExpress's PBS Buffer (10x) is filtered, free of Mg2+, Ca2+, and phenol red, and has a pH ranging from 7.2 to 7.4.
- Filtered
- Free of Mg2+
- Free of Ca2+
- Free of phenol red
- pH is 7.2-7.4
INNOVAPREP LLC Elution Fluid Can, Tris-buffered solution, Water >99.9%, Tween 20 0.075%, Carbon Dioxide propellant, 125 psi pressure, 50 extractions per can, Non-flammable gas
The InnovaPrep HC08001 Elution Fluid Can is a specially formulated Tris-buffered solution designed for efficient recovery of particles using the InnovaPrep Concentrating Pipette systems. This carbonated elution fluid employs patented Wet Foam Elution™ technology for high recovery rates of microorganisms and particles, maintaining their integrity for various analytical applications. The pressurized can format enhances laboratory workflows with its ready-to-use capability, facilitating rapid and reliable sample concentration.
- Optimized particle recovery through Wet Foam Elution™ technology
- Preserves integrity for downstream compatibility
- Supports up to 50 extractions per can for high throughput
- Pressurized can ensures safe and immediate use
Medchemexpress LLC Bes | 10191-18-1 | MFCD00007533 | 98.0% | 213.25 | 100 G
BES is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is utilized in diagnostic testing and is intended for research use only.
- Effective pH range of 6.4 to 7.8
- Utilized in diagnostic testing applications
- Purity of 98.0%
- Molecular weight of 213.25
- Appearance as a white to off-white solid
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Indofine Chemical 1,3,5-Triphenyl Tetraz, 1 Gm
1,3,5-TRIPHENYL TETRAZOLIUM FORMAZAN, Products for Life Science, 531-52-2, 1 gm