Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC HEPES Sodium salt 75277-39-3 250g
HEPES Sodium salt (CAS 75277-39-3) is a zwitterionic buffering agent designed to stabilize hydrogen ion concentration and maintain physiological pH (6 8 8 2) in biological systems thereby supporting cellular function and biomolecular interactions HEPES Sodium salt exerts its biological activity primarily through non-volatile amphoteric chemical buffering In in vitro and cell-based settings HEPES Sodium salt supports cell viability and experimental consistency by minimizing pH fluctuations Based on these properties HEPES Sodium salt holds research potential in cell-based assays maintenance of cultured mammalian cells controlled in vitro experiments studies of lysosomal biogenesis and investigations of cellular metabolic mechanisms
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 120 Grams of Powder, Makes 25 Liters of Solution
Murashige & Skoog Medium with MES Buffer is a specialized medium formulated for plant tissue culture, supplied in a 25-liter format. With 120 grams of powder, this formulation makes 25 liters of nutrient solution. It is enhanced with MES buffer, which ensures that the pH remains stable throughout the culture process, providing optimal conditions for plant cell growth. This medium is ideal for large-scale plant tissue culture studies and experiments. The MES buffer stabilizes the pH, preventing fluctuations that can affect cellular processes, making it essential for consistent growth in laboratory and research environments. This medium is commonly used in biotechnology and plant regeneration projects.
AG Scientific Inc HEPES Free Acid 1 KG
HEPES is a zwitterionic N-substituted aminosulfornic acid buffer used in cell culture and as an ampholytic separator to create a pH gradient in isoelectric focusing. It is used as a non-coordinating buffer in solutions with metal ions because it is not likely to form a complex with metal ions. The buffer is able to form free radicals so it is not suitable for redox reactions. It is reported that oxidative DNA damage can be induced by free radicals generated from HEPES buffer in the presence of Au(III).In addition, it is demonstrated that HEPES in RPMI medium potently increases hydrogen peroxide and cytotoxic products during exposure to medium to visible light.
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659982.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 250 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent and buffering agent used to prepare stable pH environments in laboratory experiments. The 250g quantity is suitable for large-scale applications and long-term use in various research projects. BES is often employed in biochemical assays, including enzyme activity studies, protein analyses, and molecular biology experiments, where maintaining a stable pH is critical. It is highly valued in protocols requiring accurate and consistent pH control, such as electrophoresis and buffer preparation for biological applications. BES is indispensable for labs focused on research that demands reliable buffering for precise biochemical reactions.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659775.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 500 G
HEPES, Sodium Salt is a biochemical reagent available in a 500g size, ideal for large-scale applications in biological and chemical research. It is widely used to maintain pH stability in biological systems, particularly in cell culture, protein assays, and enzyme reactions. HEPES sodium salt provides reliable buffering capacity in the pH range of 6.8–8.2, ensuring that experimental conditions remain consistent. It is non-toxic to cells, supporting reproducible results in long-term experiments. This bulk package is perfect for high-throughput laboratories, where large quantities of buffer are required for routine cell culture and biochemical analysis procedures.
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659941.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 500 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 500g volume is a biochemical reagent used for pH buffering in various laboratory applications, particularly where enhanced ionic strength is required. This sodium salt form is ideal for experiments that demand robust pH control, such as in cell culture, enzyme assays, and protein purification. The 500g size is ideal for medium-scale laboratory use, providing enough reagent for multiple experiments or ongoing studies. MES Sodium Salt is non-interfering, making it suitable for sensitive biological systems and molecular biology studies where maintaining a stable pH is crucial for accurate results.
Medchemexpress LLC MES monohydrate (Standard) | 145224-94-8 | MFCD00149409 | 100.0% | 25 MG
MES monohydrate (Standard) is an analytical standard and an amphoteric ion buffer used in research and analytical applications. This compound, also known as 2-Morpholinoethanesulphonic acid, is a Good's buffer with a capacity ranging from pH 5.5-7.0.
- Analytical standard for research and analytical applications
- Functions as an amphoteric ion buffer
- Offers a buffer capacity from pH 5.5-7.0
- Widely used in biochemistry and molecular biology
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Utilized in qualitative, quantitative, and methodological research experiments
- Compatible with techniques such as HPLC, GC, and MS
Medchemexpress LLC 2-Methylthioadenosine diphosphate trisodium | 475193-31-8 | 99.9% | 539.24 | 1 MG
2-Methylthioadenosine diphosphate trisodium is a potent purinergic P2Y receptors agonist. It induces platelet aggregation and shape change, and inhibits cyclic AMP accumulation in platelets exposed to prostaglandin E1.
- Potent purinergic P2Y receptors agonist
- Induces platelet aggregation and shape change
- Inhibits cyclic AMP accumulation
Medchemexpress LLC HEPES (sodium) | 75277-39-3 | MFCD00036463 | 99.9% | 260.29 | 250 MG
HEPES sodium is a nonvolatile zwitterionic chemical buffering agent broadly applied in cell culture. It is effective at pH 6.8 to 8.2 and is also a potent inducer of lysosome biogenesis.
- Nonvolatile zwitterionic chemical buffering agent
- Broadly applied in cell culture
- Effective at pH 6.8 to 8.2
- Potent inducer of lysosome biogenesis
- Maintains superhydrophilicity of titanium for at least 3 months, resulting in continuous retention of bioactivity and osteoconductivity
- Drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, and disrupts global cellular transcriptional profiles, leading to the activation of a MiT/TFE-dependent lysosomal-autophagic gene network in cultured RAW264.7 cells
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660293.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid], sodium salt, 100 G
MES Sodium Salt (2-(N-Morpholino) ethanesulfonic acid), sodium salt, in a 100g volume is a biochemical reagent designed for pH buffering in laboratory experiments. It is commonly used in biological assays, cell culture, and protein purification due to its ability to maintain a stable and non-interfering pH. The sodium salt form of MES is particularly useful in experiments where higher ionic strength is required. This 100g size is ideal for smaller experiments and research studies that need precise pH control without requiring large quantities of reagent. MES Sodium Salt is an essential component in molecular biology and biochemistry for maintaining optimal conditions in sensitive biological assays.
