Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 500g
HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture | Purity: | MW: 238.3 | 7365-45-9 | MFCD00006158 | 500g
FUJIFILM Biosciences HEPES Buffer Solution 1M
Buffer to maintain pH between 7.2 and 7.6. Suitable for use in cell cultures outside of a CO2 incubator.
Electron Microscopy Sciences D-PBS, Formula I Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences D-PBS, Formula I Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences D-PBS, Formula III Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 1 L
Synonym: TBS, 10 ×, solution. Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline | MFCD00131855 | 1L
Phosphate Buffered Saline | MFCD00131855 | 1L
Nusep Inc NUVIEW MINI GEL 4-20 12-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 4-20% 12well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Teknova 1M Tris-HCl, pH 8.0 Solution. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1M Tris-HCl, pH 9.0. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.