Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
Alkali Scientific TRIS Glycine SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
TRIS Glycine SDS Buffer (10X Powder) is a commonly used buffer solution in electrophoresis and protein separation techniques. This 184g powder is designed to make 1 liter of 10X concentrated solution, ideal for preparing protein gels for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). The buffer helps maintain a consistent pH and conductivity during protein separation, ensuring reproducible results. It is widely used in laboratories for protein analysis, molecular biology applications, and biochemistry experiments. TRIS Glycine SDS Buffer is essential for the proper functioning of electrophoresis systems, helping researchers isolate proteins based on size and charge in a reliable and efficient manner.
Hampton Research HR2-579/1.0 M TRIS hydrochloride - 100 ml
HR2-579/1.0 M TRIS hydrochloride - 100 ml. Please note items from this supplier are non-returnable.
Promocell GmbH PromoCell Dulbeccos PBS 500 ml, w/o Ca++/ Mg++
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.Dulbecco's PBS can be purchased with or without Ca^2 ^/ Mg^2 ^.
Medchemexpress LLC PBS Buffer (10x) | 1 L
Phosphate-Buffered Saline (PBS) is a balanced salt solution primarily utilized for various cell culture applications, including washing cells, diluting cells, and preparing reagents. MedChemExpress's PBS Buffer (10x) is filtered, free of Mg2+, Ca2+, and phenol red, and has a pH ranging from 7.2 to 7.4.
- Filtered
- Free of Mg2+
- Free of Ca2+
- Free of phenol red
- pH is 7.2-7.4
Seracare Life Sciences Inc 10X PHOSPHATE BUFFERED SALINE
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications. pH=7.4. Conductivity controlled.
Apexbio Technology LLC Annexin V-PE Apoptosis Kit 20tests
Annexin V-PE Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes Alternatively you can directly use our ready-to-use dual-staining kits Annexin V-PE/7-AAD Apoptosis Kit ( K2283) or Annexin V-PE/DAPI Apoptosis Kit (K2282)