Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 100 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Medchemexpress LLC Heppso | 68399-78-0 | 99.9% | 5 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- It is a zwitterionic buffer.
- Its working pH range is 7.1-8.5.
- It displays relatively high ability to bind copper(II).
- It has a pKa of 7.84 at 2.0 mM buffer concentration.
- Available in solid form, white to off-white in color.
- Store powder at -20°C for 3 years or 4°C for 2 years.
- Store in solvent at -80°C for 2 years or -20°C for 1 year.
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 25 G
MOPS sodium salt is a buffering agent utilized in biological applications. MOPS buffer is effective in maintaining the pH of mammalian cell culture media and has been shown to significantly improve the thermal stability of BSA. It is relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Buffering agent for biological research.
- Maintains pH in mammalian cell culture media.
- Significantly improves the thermal stability of BSA.
- Relatively safe for short-term experiments, though it may reduce cell viability after 4 hours, suggesting experimental duration limits of 4-12 hours.
- Can be used in the preparation of keratinocyte and microbial culture media.
Research Products International Corp HEPES, Sodium Salt, 5 KG
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
![eMolecules TERT-BUTYL 2-(HYDROXYMETHYL)-1-OXA-7-AZASPIRO[4.4]NONANE-7-CARBOXYLATE | | | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502198948.JPG-250.jpg)
eMolecules TERT-BUTYL 2-(HYDROXYMETHYL)-1-OXA-7-AZASPIRO[4.4]NONANE-7-CARBOXYLATE | | | 1g
AstaTech | TERT-BUTYL 2-(HYDROXYMETHYL)-1-OXA-7-AZASPIRO[4.4]NONANE-7-CARBOXYLATE | 1g | 624127283 | AT23569 | 95.000 | | | 257.330 | C13H23NO4
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Medchemexpress LLC NVP-TAE684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.2 g/mol | C30H40ClN7O3S | 10 MG
NVP-TAE684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in biochemical and cellular research. It blocks growth of ALK-dependent and ALCL-derived cell lines with low-nanomolar IC50 values and has been applied in preclinical studies to probe ALK signaling and validate target engagement. Typically supplied as a solid for in vitro experiments.
- Potent ALK inhibition at low-nanomolar concentrations.
- Selective activity against ALK-driven cell lines.
- Suitable for biochemical and cellular assays.
- Supplied as a solid for research use.
- Characterized with reported purity and analytical data.
Kd Medical Inc TAE (TRIS-ACETATE EDTA), 50X
TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 500ml bottle.