Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 100g
MOPS | Purity: 99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 100g
Research Products International Corp TG 10X Solution, 1 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol
Alkali Scientific QuickSilver™ Buffer Powder, TAE Buffer Powder, 100 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Boston Bioproducts Inc Molecular Biology Grade Water - 2x1L
Molecular Biology Grade Water - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4) - 2x1L
Bioprocess Grade - Phosphate Buffered Saline PBS (pH 7 4) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-P+ Buffer (10X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
ATHENA ES HBS w/Phenol Red, 500 ml
Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Boston Bioproducts Inc HBS-EP+ Buffer (10X, pH 7.4) - 4x500ml
HBS-EP Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Ethanol (20%) - 2x1L
Bioprocess Grade - Ethanol (20%) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-EP Buffer (1X, pH 7.4) - 4x500ml
HBS-EP Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-P+ Buffer (1X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)