Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![eMolecules 467454-51-9 | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158706.JPG-250.jpg)
eMolecules 467454-51-9 | tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718
tert-butyl 1-(hydroxymethyl)-2-azabicyclo[2.1.1]hexane-2-carboxylate | Pharmablock | 467454-51-9 | MFCD24466941 | 213.277 | C11H19NO3 | 97.000 | CC(C)(C)OC(=O)N1CC2CC1(CO)C2 | 1g | 586155718
Crescent Chemical Co Inc SERVA TRIS-TRICINE/SDS BUFFER
SERVA Tris-Tricine/SDS electrophoresis buffer (10x) Running buffer for SDS PAGE. Supplied as 10 x concentrate. Contains 1 M Tris, 1 M Tricine and 1 % SDS. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42552.01
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 1L Stor
Alkali Scientific Tris Acetate Ultra Pure, 500 G
Tris Acetate Ultra Pure is a high-quality buffering agent formulated for high-precision laboratory applications. With its ultra-pure grade, it ensures minimal interference in sensitive experiments, maintaining a stable pH environment. This 500g bottle is ideal for medium-scale preparations, supporting a wide range of biochemical and molecular biology applications, including electrophoresis, protein assays, and enzyme reactions. The tris acetate buffer is particularly effective for experiments requiring consistent pH conditions, making it a reliable choice for researchers working in protein analysis, DNA/RNA extraction, and various other scientific fields where precision is critical.
Bioworld TTE Buffer, Ready-Pack (20X), 1 PK(s)
20x TTE (Tris-TAPS-EDTA) buffer pack can be dissolved into 2L of water to make 2L of 1x TTE solution.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 9.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 9.5 (0.2 micron filtered), 500 ml.
American Research Products Inc TRIS-ACETATE BUFFER, 0.2M,
TRIS-Acetate Buffer, 0.2M, pH 7.8-500ml. 0.2M Tris base dissolved in 18.2 megohms water, pH adjusted to 7.8 with glacial acetic acid and filtered through 0.22-micron filter.
Biotang Inc Tris-Glycine Native Sample Buffer (4X), 25 ml
Tris-Glycine Native Sample Buffer (4X), 25 ml.