Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 1L
Tris-HCl Buffer (1 M pH 8 0) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 500ml
HEPES Buffer (1 M pH 7 6 pH Adjusted with KOH) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.0) - 250ml
Bicine Buffer (0 5 M pH 8 0) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 1L
Tris-HCl Buffer (1 M pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.6) - 1L
Tris Buffered Saline TBS (10X pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences Hanks Balanced Salts Modif1L
HANKS Hanks Balanced Salts Modif1L
Electron Microscopy Sciences Hepes, Crystal, Biological Buffer, 7.0-8.0 pH 100 GR
(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
CAS #7365-45-9 C₈H₁₈O₄N₂S F.W. 238.30
Assay: >99%
Good's buffer.
pH: 7.0-8.0
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences MOPS-EDTA-Sodium Acetate M1L
MOPS - EDTA - Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum reSOLUTIONon.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate | 1266615-59-1 | MFCD00149409 | 100 g
MES hydrate | Purity: 99.5% | Mol Wt: 195.24 | 1266615-59-1 | MFCD00149409 | 100 g
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerforman1L
MOPS SOLUTIONon BioPerforman1L
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline liquid, sterile-filtered, DPBS Modified, without calcium, without magnesium, suitable for cell culture | MFCD00131855 | 1000ML
Dulbecco's Phosphate Buffered Saline liquid, sterile-filtered, DPBS Modified, without calcium, without magnesium, suitable for cell culture | MFCD00131855 | 1000ML
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 1 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1