Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc HEPES Free Acid 100 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES w/oL-Glutamine is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt 99.5 tit1KG
HEPES sodium salt 99.5 tit1KG
Medchemexpress LLC Bes | 10191-18-1 | MFCD00007533 | 98.0% | 213.25 | 100 G
BES is a secondary standard biochemical buffer with an effective pH range of 6.4 to 7.8 (pKa: 7.15 at 20 °C). It is utilized in diagnostic testing and is intended for research use only.
- Effective pH range of 6.4 to 7.8
- Utilized in diagnostic testing applications
- Purity of 98.0%
- Molecular weight of 213.25
- Appearance as a white to off-white solid
Sigma Aldrich Fine Chemicals Biosciences HEPBS >=99% (titration) | 161308-36-7 | MFCD00677124 | 100G
HEPBS >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 266.36 | 161308-36-7 | MFCD00677124 | 100G
Electron Microscopy Sciences Hepes, Crystal, Biological Buffer, 7.0-8.0 pH 25 GR
(N-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic acid)
CAS #7365-45-9 C₈H₁₈O₄N₂S F.W. 238.30
Assay: >99%
Good's buffer.
pH: 7.0-8.0
Boston Bioproducts Inc Krebs-Henseleit Buffer (with HEPES, without Calcium) - 500ml
Krebs-Henseleit Buffer (with HEPES without Calcium) - 500ml (For Research Use and Further Manufacturing Only)
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 50G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 50G
![Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40140-5000.0.JPG-250.jpg)
Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5