Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
Trifluoroacetic Acid, Reagents
CAS: 76-05-1 Molecular Formula: C2HF3O2 Molecular Weight (g/mol): 114.02 MDL Number: MFCD00004169 InChI Key: DTQVDTLACAAQTR-UHFFFAOYSA-N Synonym: Perfluoroacetic Acid, Trifluoroethanoic Acid IUPAC Name: trifluoroacetic acid SMILES: OC(=O)C(F)(F)F
Trifluoroacetic Acid, For protein sequencing, Solstice
CAS: 76-05-1 Molecular Formula: C2HF3O2 Molecular Weight (g/mol): 114.02 MDL Number: MFCD00004169 InChI Key: DTQVDTLACAAQTR-UHFFFAOYSA-N Synonym: trifluoroacetic acid,perfluoroacetic acid,trifluoroethanoic acid,trifluoroacetic acid,acetic acid, trifluoro,trifluoroaceticacid,cf3cooh,trifluoro acetic acid,trifluoro-acetic acid,kyselina trifluoroctova PubChem CID: 6422 ChEBI: CHEBI:45892 IUPAC Name: trifluoroacetic acid SMILES: OC(=O)C(F)(F)F
Acetonitrile with 0.1% Trifluoroacetic acid, LabReady™ Solvent Blend, Solstice
CAS: 75-05-8 Molecular Formula: C2H3N Molecular Weight (g/mol): 41.053 InChI Key: WEVYAHXRMPXWCK-UHFFFAOYSA-N Synonym: methyl cyanide,cyanomethane,ethanenitrile,methanecarbonitrile,ethyl nitrile,methane, cyano,acetonitrile,cyanure de methyl,methylkyanid,mecn PubChem CID: 6342 ChEBI: CHEBI:38472 IUPAC Name: acetonitrile SMILES: CC#N
Acetonitrile with 0.035% Trifluoroacetic acid, LabReady™ Solvent Blend, Solstice
CAS: 75-05-8 Molecular Formula: C2H3N Molecular Weight (g/mol): 41.053 InChI Key: WEVYAHXRMPXWCK-UHFFFAOYSA-N Synonym: methyl cyanide,cyanomethane,ethanenitrile,methanecarbonitrile,ethyl nitrile,methane, cyano,acetonitrile,cyanure de methyl,methylkyanid,mecn PubChem CID: 6342 ChEBI: CHEBI:38472 IUPAC Name: acetonitrile SMILES: CC#N
Sigma Aldrich Fine Chemicals Biosciences Trifluoroacetic acid ReagentPlus(R), 99% | 76-05-1 | MFCD00004169 | 25ML
Trifluoroacetic acid ReagentPlus(R), 99% | Purity: 99% | Mol Wt: 114.02 | 76-05-1 | MFCD00004169 | 25ML
Proteochem Inc TFA Ampules (Proteomics Grade), 76-05-1, MFCD00004169, 10 amps
MF: C2 H F3 O2, MW: 114.03, Alternative Names: TFA Ampoules; Trifluoroacetic Acid Ampoules; TFA Amps. Trifluoroacetic acid (TFA) is an effective ion pairing agent for HPLC and proteomics LCMS applications which are regularly used to resolve chromatographically similar analytes.
Medchemexpress LLC Ms15 TFA | C66H80F3N11O7S | 5 MG
MS15 TFA is a potent and selective AKT PROTAC degrader for research use only. It effectively inhibits AKT1, AKT2, and AKT3 activities, demonstrating bioavailability in mice through intraperitoneal administration.
- Potent and selective AKT PROTAC degrader
- Inhibits AKT1, -2, and -3 activities
- IC50 values: 798 nM (AKT-1), 90 nM (AKT-2), 544 nM (AKT-3)
- Bioavailable in mice via intraperitoneal administration
- Purity of 99.82%
Medchemexpress LLC Astax-35r TFA | 99.1% | 2392.86 | 5 MG
aStAx-35R TFA is a stapled peptide that antagonizes the nuclear form of β-catenin and inhibits Wnt signaling. It competitively inhibits the binding of β-catenin to TCF4 and selectively induces growth inhibition of Wnt-dependent cancer cells.
- Antagonizes nuclear form of β-catenin.
- Inhibits Wnt signaling.
- Competitively inhibits the binding of β-catenin to TCF4.
- Selectively induces growth inhibition of Wnt-dependent cancer cells.
Medchemexpress LLC Wrwyar-NH2 TFA | 936.07 | 1 MG
Wrwyar-NH2 (TFA) is a control peptide.
- Control peptide for wrwycr-NH2
- Targets DNA/RNA synthesis
- Involved in the cell cycle/DNA damage pathway
Medchemexpress LLC CPP12 (TFA) | 98.7% | 1700.55 | 5 MG
CPP12 TFA is a small, amphiphilic, cyclic cell-penetrating peptide (CPP) in salt form. It binds directly to plasma membrane phospholipids, enters mammalian cells via endocytosis, and is then efficiently released from endosomes. This peptide can be used for intracellular delivery of drugs and chemical probes.
- Small, amphiphilic, cyclic cell-penetrating peptide
- Binds directly to plasma membrane phospholipids
- Enters mammalian cells via endocytosis
- Efficiently released from endosomes
- Used for intracellular delivery of drugs and chemical probes
Medchemexpress LLC Cys-PKHB1 TFA | 1487.88 | 10MG
Cys-PKHB1 TFA is a peptide conjugated to PKHB1, functioning as a CD47 agonist peptide and a thrombospondin-1 peptide mimic with antitumor effects. It induces mitochondrial alterations, reactive oxygen species generation, and intracellular calcium accumulation, leading to calcium-dependent cell death in breast cancer cells. This compound also activates the immune system in breast cancer cells via immunogenic cell death.
- Conjugated peptide acting as a CD47 agonist
- Thrombospondin-1 peptide mimic
- Exhibits antitumor effects
- Induces mitochondrial alterations
- Promotes reactive oxygen species (ROS) generation
- Causes intracellular calcium (Ca+) accumulation
- Leads to calcium-dependent cell death in breast cancer cells
- Activates the immune system through immunogenic cell death
Medchemexpress LLC Ampreloxetine TFA | 1227056-85-0 | 98.3% | 25 MG
Ampreloxetine TFA (trifluoroacetate) is the TFA salt of ampreloxetine (TD-9855), an orally active inhibitor of norepinephrine and serotonin uptake supplied as a solid research reagent. It is intended for in vitro and preclinical research and is provided in small package sizes with recommended refrigerated storage.
- Potent norepinephrine and serotonin uptake inhibitor.
- Trifluoroacetate (TFA) salt, solid form.
- Purity 98.3%.
- Molecular weight 435.36 g/mol.
- Available in small research sizes, including 25 mg.
- Storage: 4°C sealed; in solvent: -80°C (6 months), -20°C (1 month).