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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (35,890)
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Search Within Results
Keyword Search:
1 – 15 of 34,943 results
1

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
GSA_VA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
2

Thermo Scientific™ Accucore™ C18 HPLC Columns
GSA_VA

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

EDGE
Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 9%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
pH 1 to 11
Surface Area 130 m²/g
Particle Shape Solid Core
USP Type L1
3

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
GSA_VA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

EDGE
Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
4

Thermo Scientific™ MAbPac™ Reversed Phase HPLC Columns
GREENER_CHOICE

Use the new Thermo Scientific™ MAbPac™ RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins.

EDGE
Pore Size 1500 Å
Particle Size 4 μm
Phase Reversed Phase
Max. Pressure 4000 psi (275 bar)
5
Promotions Available

Thermo Scientific™ DNAPac™ RP Columns
GREENER_CHOICE

Get high resolution LC and LC-MS separation of oligonucleotides, including double-stranded (ds) DNA/RNA fragments, using these DNAPac reversed phase columns.

EDGE
6
Promotions Available

Thermo Scientific™ MAbPac™ SEC-1 Size Exclusion Chromatography HPLC Columns
GREENER_CHOICE

Use these SEC columns for monoclonal antibody (mAb) analysis, including the high-resolution separation of monomers, aggregates and fragments.

Pore Size 300 Å
Particle Size 5 μm
Solvents 100% Organic Solvents
Temperature 30°C
Phase Size Exclusion
Column Type Size Exclusion
pH 2.5 to 7.5
Quantity Each
Product Line MAbPac
7

MilliporeSigma™ Supelco™ Ascentis™ Si HPLC Column, 3 μm Particle Size

The Ascentis™ Si is a high loading capacity silica with excellent peak shape. The Ascentis™ Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase SIlica
Phase Reversed Phase
Matrix Silica Gel High Purity, Spherical/Silica Phase
Endcapped No
pH 2 to 6
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
8

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18 HPLC Column, 3 μm Particle Size

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Pore Size 120 Å
Particle Size 3 μm
Stationary Phase C18 (Octadecyl)
Phase Reversed Phase
Matrix C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
9

TSKgel™ ODS-100Z HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ ODS-100Z column's bonded phases are prepared by reacting the silica surface with a difunctional octadecylsilane reagent, followed by repeated endcapping with a monofunctional trimethylsilane reagent.

Particle Size 3 μm
Solvents 0 to 100% organic solvent
Carbon Load 20%
Stationary Phase C18 (octadecyl)
Temperature 10°C to 50°C
Phase Reversed Phase
Matrix Spherical Silica
pH 2.0 - 7.5
Material Stainless Steel
Surface Area 450 m2/g
Product Line TSKgel
USP Type L1
10

MilliporeSigma BIOshell A120 Oligo C18 (2.7 μm) HPLC Column

The HPLC Column contains 1 Pk.

Pore Size 120 Å
Particle Size 2.7 μm
Phase Reversed Phase
Product Type HPLC Column
Diameter (Metric) 1.5 mm
Material Stainless Steel
Technique HPLC
11

MilliporeSigma™ Supelco™ Ascentis™ Express C18 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Stationary Phase C18 (octadecyl)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Material Stainless Steel
Max. Pressure 600 bar(9000 psi)
Column Format Analytical Column
Particle Size 2.7 μm
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Product Type HPLC/UHPLC Column
Packing Material Fused-Core particle platform superficially porous particle
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
USP Type USP L1
12

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å AQ-C18 HPLC Column, 2.7 μm Particle Size

A high-speed, high-performance liquid chromatography column based on the Fused-Core™ particle design.

Pore Size 90 Å
Particle Size 2.7 μm
Carbon Load 6.7%
Phase Reversed Phase
Matrix AQ-C18 Phase/Fused-Core™ Silica Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Surface Area 135 m2/g
Product Line Ascentis
USP Type L1
13

MilliporeSigma™ Supelco™ Ascentis™ Express HILIC HPLC Column, 5 μm Particle Size

The bare silica stationary phase of Ascentis™ Express 5 μm HILIC provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
14

TSKgel™ GMPW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ GMPW is a mixed bed scouting column for water-soluble linear polymers. The pore volume can be accessed by polymers ranging in molecular weight from 500 to 8,000,000 Da.

Particle Size 17 μm
Solvents ≤20% organic solvent
Stationary Phase Hydroxylated Methacrylate
Temperature 80°C
Phase Size Exclusion
Matrix Polymer Particle Platform
Diameter (Metric) 7.5 mm
pH 2.0 - 12
Product Line TSKgel
USP Type L38, L39
15
Promotions Available

Thermo Scientific™ Acclaim™ Mixed Mode WCX-1 HPLC Columns
GSA_VA GREENER_CHOICE

Separate cationic molecules with powerful adjustable selectivity control using this mixed-mode reversed-phase and weak cation-exchange LC column.

EDGE
Pore Size 120 Å
Stationary Phase Hydrophobic Alkyl Chain with a Carboxyl Terminus
Temperature 50°C
Phase Reversed Phase, Ion Exchange, Ion Exclusion, HILIC
Endcapped Proprietary
Column Type Mixed Mode
Green Features FSC
Surface Area 300 m²/g
Product Line Acclaim
USP Type L85