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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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1 – 15 of 30,967 results
1

Thermo Scientific™ Hypersil™ ODS C18 Columns
GSA_VA

Use as a general purpose C18 phase for acidic and neutral analytes.

EDGE
Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
2

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
3

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
GSA_VA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
4

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
GSA_VA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

EDGE
Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
5

Thermo Scientific™ Accucore™ C18 HPLC Columns
GSA_VA

Use this column, with its specially designed solid core, for a broad range of nonpolar analytes across multiple applications.

EDGE
Pore Size 80 Å
Particle Size 2.6 μm
Carbon Load 9%
Stationary Phase C18
Temperature 70°C
Phase Reversed Phase
Endcapped Yes
pH 1 to 11
Surface Area 130 m²/g
Particle Shape Solid Core
USP Type L1
6

Thermo Scientific™ ProSwift™ RP-1S PSDVB Reversed Phase HPLC Column, Monolith, 4.6 mm x 50 mm
GREENER_CHOICE

Column Format: Analytical Column; Pore Size: Monolith; Column Phase: Reversed Phase

EDGE
7

Thermo Scientific™ Hypersil™ APS-2 Amino (NH2) Mixed Mode HPLC Column, 5 μm, 4 mm x 300 mm

Column Format: Analytical Column; Carbon Load: 1.90%; Pore Size: 120 Å; Column Phase: Reversed Phase, Normal Phase, Ion Exchange, HILIC

8

Thermo Scientific™ Hypersil GOLD™ PEI HILIC HPLC Columns
GSA_VA

Retain highly polar and hydrophilic analytes, which are difficult to analyze with conventional C18 columns, using Hypersil GOLD PEI HILIC LC Columns.

Pore Size 175 Å
Carbon Load 6%
Stationary Phase PEI HILIC
Temperature 60°C
Phase HILIC
Endcapped No
pH 2 to 8
Surface Area 220 m²/g
9

MilliporeSigma™ Supelco™ Ascentis™ RP-Amide HPLC Column, 3 μm Particle Size

Ascentis™ RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis™ RP-Amide is the first choice in embedded polar group HPLC phases.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase Amide, Alkyl
Phase Reversed Phase
Matrix Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
10

Thermo Scientific™ ProPac™ 3R SAX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SAX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and AAVs.

Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Proprietary
Temperature Ambient to 60°C
Phase Strong Anion Exchange
Packing Material Monodisperse Polymer Resin
Column Type Ion Exchange
pH 2 to 12
Technique Bio-LC
Max. Pressure 4500 psi (310 bar)
Product Line ProPac
Column Format Analytical Column
11

Thermo Scientific™ ProPac™ 3R SCX 3 μm HPLC Columns

Get the performance you need with ProPac™ 3R SCX HPLC columns. Suitable for charge variant separations and recommended for therapeutic proteins, mAbs, ADCs, and biosimilar proteins.

Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Proprietary
Temperature Ambient to 60°C
Phase Strong Cation Exchange
Packing Material Monodisperse Polymer Resin
Column Type Ion Exchange
Mobile Phase Compatibility Recommended for use with CX-1 pH buffers
pH 2 to 12
Technique Bio-LC
Max. Pressure 4500 psi (310 bar)
Product Line ProPac
Column Format Analytical Column
12

Thermo Scientific™ Hypersil™ Chiral HPLC Columns

Resolve enantiomers with Hypersil Chiral HPLC columns in normal phase mode for confident purification of your target compound.

Pore Size 1000 Å
Temperature Room Temperature
Packing Material Spherical, Fully Porous Silica
Surface Area 30 m²/g
Max. Pressure 3700 psi (255 bar)
Column Format Preparative Column and Analytical Column, Standard
13

Thermo Scientific™ Shodex™ SEC/GPC Phase Mixed Mode HPLC Column, 3 μm, 4.6 mm x 300 mm
GSA_VA

Column Format: Semi-Micro Column; Pore Size: 800 Å; Column Phase: Size Exclusion and Gel-Permeation

14

Thermo Scientific™ Hypersil™ PREP HS C18 HPLC Columns

Maximize retention and loading with Hypersil PREP HS C18 prep LC reversed phase columns which offers high loading capacity and suitable for USP-L1 Applications.

Pore Size 100 Å
Carbon Load 15%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 350 m²/g
USP Type L1
15

Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

EDGE
Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column