Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,619)

Singleplex and Multiplex Bead Based Immunoassays (6,174)

ELISA Substrates and Reagents (2,341)

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286 – 300 of 138,521 results

286

Invitrogen™ Mouse IL-6 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	Il6
Total Assay Time	24 hr 30 min
Sample Volume	Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16193
Sample Type	Cell Lysates,Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-6

287

Invitrogen™ Human IL-1 beta Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IL1B
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2-8°C
Assay Range	2-150 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P01584
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3553
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-1 beta

288

Invitrogen™ Human IL-1 beta Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IL1B
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2-8°C
Assay Range	2-150 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P01584
Regulatory Status	RUO
Target	IL-1 beta
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3553
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Interleukin-1 beta

289

Invitrogen™ Human IgE Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	7.8 ng/mL
Assay Range	7.8-500 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Regulatory Status	RUO
Target	Human IgE
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Human IgE protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Human IgE protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3497
Sample Type	Plasma,Serum
Synonym	Immunoglobulin E

290

Invitrogen™ Human Calprotectin L1/S100-A8/A9 Complex ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	S100A8, S100A9
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	35 pg/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	35-8000 pg/mL
Test Time	1 hr 20 min
Accession Number	P05109, P06702
Regulatory Status	RUO
Target	Calprotectin
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	6279, 6280
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

291

Invitrogen™ Human GDF15 ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Family	Growth Factors & Receptors
Target Species	Human
Conjugate	HRP
Intraassay CV	<10%
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Research Discipline	Signal Transduction
For Use With (Application)	ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time	1 hr 20 min
Assay	Solid-phase sandwich ELISA
Target	GDF15
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Label or Dye	Biotin
Calibration Range	2 pg/mL to 800 pg/mL
Interassay CV	<12%
Gene Symbol	Gdf15
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	2-800 pg/mL
Accession Number	Q99988
Regulatory Status	RUO
Product Type	ELISA
Gene ID (Entrez)	9518
Sample Type	Plasma,Serum,Supernatant
Synonym	GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB

292

Invitrogen™ Ceruloplasmin Colorimetric Activity Kit

Assay Ceruloplasmin Activity in Serum and Urine samples.

Pricing & Availability

293

Invitrogen™ 20-Hydroxyecdysone ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	9%
Intraassay CV	10%
Total Assay Time	2 hr 30 min
Sample Volume	Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
Assay Sensitivity	197.8 pg/mL
Storage Requirements	2-8°C
Assay Range	102.4-25000 pg/mL
Test Time	45 min
Accession Number	20 E; C27H44O7; ecdysterone
Regulatory Status	RUO
Target	20-Hydroxyecdysone
Kit Contents	Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer,Standard,Assay Buffer Concentrate,Capture Antibody,HRP-conjugated Antigen,Wash Buffer Concentrate,TMB Substrate,Stop Solution,Plate Sealer
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Fecal Extract,Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

294

Invitrogen™ Thiol Fluorescent Detection Kit

Assay Thiol levels in protein and peptide containing biological buffers including Tris, phosphate, and citrate buffers.

Pricing & Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Gene ID (Entrez)	Non-Gene
Gene Symbol	Non-Gene
Target Kit Named	Thiol

295

Invitrogen™ Human IgG (Total) ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	2.6%
Intraassay CV	5.3%
Total Assay Time	1 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	0.24 ng/mL
Storage Requirements	2-8°C
Assay Range	1.6-100 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Human IgG
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum
Label or Dye	HRP
Synonym	Immunoglobulin G

296

Invitrogen™ Mouse IL-10 Instant ELISA™ Kit

ELISA

Pricing & Availability
Specifications

Protein Aliases	IL-10, Cytokine synthesis inhibitory factor, CSIF
Immunoassay Kit Format	Coated ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	10.1%
Intraassay CV	6.7%
Gene Symbol	IL10
Total Assay Time	3 hr 10 min
Assay Sensitivity	5.28 pg/mL
Storage Requirements	-20°C
Assay Range	39.1-2500 pg/mL
Test Time	15 min
Accession Number	P18893
Regulatory Status	RUO
Target	IL-10
Kit Contents	Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers,Biotinylated Detection Antibody,SAV-HRP,Sample Diluent,lyophilized,Standard (8-well strips x 4),Wash Buffer Concentrate,Substrate Solution,Sample Diluent,Stop Solution,Lysis Buffer 10x,Adhesive Plate Covers
Gene Alias	CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIFCSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF; CSIF; IL-10
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	16153
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

297

Invitrogen™ Human IgE ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.2%
Intraassay CV	6.6%
Total Assay Time	1 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL
Assay Sensitivity	0.5 ng/mL
Storage Requirements	2-8°C
Assay Range	7.8-500 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Human IgE
Kit Contents	Pre-coated 96 well plate, Standard, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer Concentrate, TMB Substrate Solution, Stop Solution, Controls, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3497
Sample Type	Plasma,Serum
Label or Dye	HRP
Synonym	Immunoglobulin E

298

Invitrogen™ Human IL-2 ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Aliases	IL-2, T-cell growth factor, TCGF
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	5%
Intraassay CV	7%
Gene Symbol	IL2
Total Assay Time	3 hr 30 min
Assay Sensitivity	9.1 pg/mL
Storage Requirements	2-8°C
Assay Range	18.8-1200 pg/mL
Test Time	1 hr 20 min
Accession Number	P60568
Regulatory Status	RUO
Target	IL-2
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Controls, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Controls,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	aldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGFaldesleukin; cytokine; H-IL-2; il 2; Il2; Il-2; ILN; Interleukin; interleukin 2; interleukin 2 precursor; interleukin 2 variant IL2delta2; Interleukin2; Interleukin-2; interleukin-2 precursor; interleukin-2 precursor protein; interleukin-2; interleukin 2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; lymphokine; M-IL-2; patial sequence of Interleukin-2; POIL2; porcine Interleukin 2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF; IL-2; TCGF; lymphokine
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	3558
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

299

Invitrogen™ ProcartaPlex™ Mouse Th1/Th2 Cytokine & Chemokine Panel 1, 20plex

The ProcartaPlex Mouse Th1/Th2 Cytokine & Chemokine Panel 1 20plex enables the study of immune function by analyzing 20 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

300

Invitrogen™ ProcartaPlex™ Porcine Cytokine & Chemokine Panel 1, 9plex

The ProcartaPlex Porcine Cytokine & Chemokine Panel 1 9plex enables the study of immune response by analyzing 9 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

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