Protein Method Blocking Buffers
LICOR Intercept T20 (PBS) Protein-Free Antibody Diluent (3x500mL)
Intercept T20 (PBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
LICOR Intercept (PBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Genetex Inc Trident Universal Protein Blocking Reagent (animal serum free)
The Trident Universal Protein Blocking Reagent (animal serum free) is in a ready to use format. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.
Research Products International Corp PBS/Tween 20 10X Solution, 500 Milliliters
PBS/Tween 20 10X Solution is a widely used buffer solution in the life science marketplace. It is a combination of phosphate-buffered saline (PBS) and Tween 20, a nonionic detergent.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 60 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry.
LAB BIOREAGENTS StrongBlock III Buffer 250ml
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
Biogenex Laboratories Power Block
Power Block ™ reagent is a highly effective blocking reagent capable of reducing non-specific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting. Antibodies tend to bind non-specifically to membranes and tissues. To minimize this nonspecific binding, a blocking buffer is used to enhance signal-to noise ratio. Power Block™ reagent shows no cross-reactivity and it blocks more effectively than serum albumins. The blocking potency of Power Block™ reagent is superior to most other conventional formulations in producing a high signal-to-noise ratio. Power Block™ reagent may also be used as effective diluents for antibodies.""""
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Rockland Immunochemicals BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 15 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry
Chondrex Inc ChonBlock Blocking/Sample Dilution ELISA Buffer 100mL
100 mL of a mammalian protein-based ELISA buffer for ELISA plate blocking or ELISA sample dilution that eliminates background noise reactions caused by non-specific reactions of antibodies in serum/plasma samples. Please visit www.chondrex.com or email Chondrex, Inc. at support@chondrex.com for more information on how ChonBlock can improve your ELISA results. pH 7.8. Contains 0.4% Tween-20 and 0.04% ProClin 300.