Protein Method Blocking Buffers
Biotang Inc BLOTTO Antifoam In PBS (with azide), 250 ml
BLOTTO Antifoam In PBS (with azide), 250 ml.
Biotang Inc BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Biotang Inc BLOTTO/Tween 20 in TBS (with azide), 250 ml
BLOTTO/Tween 20 in TBS (with azide), 250 ml.
Biotang Inc BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO/TWEEN 20 IN TBS 250 ML, IBB-161. 5% nonfat dry milk, 0.05% Tween-20 in TBS (50 mM Tris-HCl and 150 mM NaCl, pH 7.4 +/- 0.2). Prepared in 18.2 megohms water and filtered through 1 micron filter.
Biotang Inc GELATIN- Blocking Buffer in PBS (with azide), 500 ml
GELATIN- Blocking Buffer in PBS (with azide), 500 ml.
Amsbio LLC ChonBlock™ Blocking/Sample Dilution ELISA Buffer- 100 ml
ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml
Biotang Inc BSA- Blocking Buffer in TBS and Tween-20, 250 ml
BSA- Blocking Buffer in TBS and Tween-20, 250 ml.
Chondrex Inc ChonBlock Detection Antibody Dilution Buffer, 100ml
100 mL of a mammalian protein-based ELISA buffer for dilution of detection/secondary antibody solutions. Please visit www.chondrex.com or email Chondrex, Inc. at support@chondrex.com for more information on how ChonBlock can improve your ELISA results. pH 7.8. Contains 0.4% Tween-20 and 0.04% ProClin 300.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 6/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010
Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
FOCUS BIOMOLECULES Biochemical Reagents, C2230, Cav2.2 channel blocker, CAS 882243-88-1, 98% by TLC/NMR (Conforms)
Biochemical Reagents, C2230, Cav2.2 channel blocker, CAS 882243-88-1, 98% by TLC/NMR (Conforms), C2230 is a novel Cav2.2 channel blocker (IC50 1.3 uM at -50 mV/ 10.2 uM at -80 mV) with a unique binding mode (no GPCR-mediated inhibition). It displays preferential binding to the inactive state of the channel. C2230 is active in both rat and human DRG neurons. Active in rat models of neuropathic pain and mouse models of osteoarthritis. C2230 does not affect somatosensation or motor and cardiac function.
FOCUS BIOMOLECULES Biochemical Reagents, MitoBloCK-10, TIMM44 blocker, CAS 394694-98-5, 98% by TLC/NMR (Conforms)
Biochemical Reagents, MitoBloCK-10, TIMM44 blocker, CAS 394694-98-5, 98% by TLC/NMR (Conforms), MitoBloCK-10 is a first in class TIMM44 blocker. Translocase of inner mitochondrial membrane 44 (TIMM44) is essential for maintaining function and integrity of mitochondria.1 It induced mitochondrial dysfunction and suppressed angiogenic activity in endothelial cells.1 Targeting TIMM44 by MitoBloCK-10 significantly inhibits bladder cancer cell growth in vitro and in vivo.2