Protein Method Blocking Buffers
Bioworld ELISA Reaction Buffer, 50mM Carbonate-Bicarbonate, 1 L
Product DescriptionELISA reaction buffer has a 50 mM concentration of carbonate-bicarbonate and a pH level of 9.6. It contains 1.59 g Na2CO3, 2.93 g NaHCO3, and dissolved in 1 liter of deionized water.Carbonate/bicarbonate buffer at a pH greater than 9 is used for the attachment of an antigen or of an antibody onto an ELISA plate. Direct coating of ELISA is an antigen immobilization technique, in which antigens are directly attached to the plate by passive adsorption. Most but not all proteins bind tightly to the polystyrene surface of microplates in alkaline conditions. Indirect coating of ELISA is another antigen immobilization technique in which antigen-specific antibodies are adsorbed onto the plastic, which in turn binds and immobilizes the antigen upon incubation with the antigen sample. Both techniques typically use the same buffer.
LICOR Intercept T20 (TBS) Protein-Free Antibody Diluent (500 mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Abcam He x amethonium bromide, nicotinic receptor blocker, 1G
MW 362.19 Da. Ganglionic blocker; antagonist at α₃-containing nicotinic acetylcholine receptors (autonomic ganglia).
The product is subject to the following: Abcam Restricted Use Statement
LICOR Intercept T20 (PBS) Antibody Diluent (10 x 500 mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Biotang Inc CASEIN- Blocking Buffer in PBS (with azide), 250 ml
CASEIN- Blocking Buffer in PBS (with azide), 250 ml.
Biolegend Tris-Buffered Saline 20X (1.0 M) w/ Tween 20
Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 Apps: IHC; Size: 500 mL
Biotium TrueBlack WB Blocking Buffer Kit
The TrueBlack WB blocking buffer is a buffer system designed to achieve optimal specificity and sensitivity by blocking background from the non-specific interaction between the fluorophore-labeled antibodies and the membrane with cell lysates. For blocking and antibody dilution for 50 membranes.
Rockland Immunochemicals BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
Abcam Capsazepine, TRPV1 antagonist, 50MG
MW 376.9 Da, Purity >98%. TRPV1 vanilloid receptor antagonist (IC₅₀ = 562 nM). Analgesic in vivo.
The product is subject to the following: Abcam Restricted Use Statement
LICOR Intercept (PBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Biotang Inc BLOTTO/Tween 20 in TBS (with azide), 250 ml
BLOTTO/Tween 20 in TBS (with azide), 250 ml.
Cell Signaling Technology Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
FOCUS BIOMOLECULES Biochemical Reagents, MitoBloCK-10, TIMM44 blocker, CAS 394694-98-5, 98% by TLC/NMR (Conforms)
Biochemical Reagents, MitoBloCK-10, TIMM44 blocker, CAS 394694-98-5, 98% by TLC/NMR (Conforms), MitoBloCK-10 is a first in class TIMM44 blocker. Translocase of inner mitochondrial membrane 44 (TIMM44) is essential for maintaining function and integrity of mitochondria.1 It induced mitochondrial dysfunction and suppressed angiogenic activity in endothelial cells.1 Targeting TIMM44 by MitoBloCK-10 significantly inhibits bladder cancer cell growth in vitro and in vivo.2
Bioworld TBS/Fish Gelatin (10X), 200 mL
TBS fish gelatin solution used as blocking, diluents and coating solutions is supplied as 10X concentrate of 5% solution.Technical Information:5% Fish gelatin in TBS bufferpH range: 7.6 +/- 0.1Sold as 2 x 100mL