Protein Method Blocking Buffers
Alkali Scientific Blotto Tween 20 Blocking Solution in TBS with Azide, 500 Milliliters
Blotto Tween 20 Blocking Solution in TBS with Azide is a pre-prepared blocking reagent commonly used in immunoassays such as Western Blot, ELISA, and immunohistochemistry. The addition of Azide ensures that the solution remains free of microbial contamination during storage. This solution blocks non-specific binding sites, ensuring that antibodies or proteins of interest bind specifically to their targets, enhancing the accuracy of experimental results. Available in a 500-milliliter bottle, it is the perfect size for routine use in laboratories performing protein analysis or other immunological studies, helping to reduce background noise and improve assay sensitivity.
Cell Signaling Technology Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
LICOR Intercept T20 (TBS) Protein-Free Antibody Diluent (500 mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Genetex Inc Trident Universal Protein Blocking Reagent (animal serum free)
The Trident Universal Protein Blocking Reagent (animal serum free) is in a ready to use format. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 500MG
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 15 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry
LICOR Intercept (PBS) Blocking Buffer (10 x 500mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Research Products International Corp BSA Block Buf Sol wAzide 200ml
BSA Blocking Buffer Solution in TBS with Azide, 200 Milliliters
LICOR Intercept (PBS) Blocking Buffer (500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Teknova 1X PBS with 0.05% Tween-20, pH 7.4. 20L Carboy, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific TBS / Tween 20 10X Solution, 500 Milliliters
TBS / Tween 20 10X Solution is a highly effective washing buffer used to minimize background noise during immunodetection procedures such as Western blotting and ELISA. This 500mL solution is ideal for smaller-scale applications or as a concentrate that can be diluted for use in various experimental setups. The Tris component provides pH stability, while Tween 20 aids in reducing non-specific binding during protein-protein interactions. This formulation ensures cleaner results by washing away excess proteins and antibodies that do not bind specifically to the target. TBS-Tween 20 is essential for improving the signal-to-noise ratio in sensitive immunoassays and diagnostic tests.
Medchemexpress LLC KCC2 blocker 1 | 1228439-36-8 | 98.2% | 415.50 | 1 ML
KCC2 blocker 1 is an orally active and selective K+-Cl- cotransporter KCC2 blocker with an IC50 of 1 μM. It is identified as a benzyl prolinate. This compound selectively inhibits KCC2, making it a valuable tool for neuronal signaling research.
- Orally active.
- Selective K+-Cl- cotransporter KCC2 blocker.
- Exhibits an IC50 of 1 μM for KCC2.
- Classified as a benzyl prolinate.
- Demonstrates antagonist activity against NKCC1 in MDCK cells.
- Applicable for research purposes.