Protein Method Blocking Buffers
Biotium Permeabilization and Blocking Buffer (5X)
Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer for use with anti-goat and anti-sheep secondary antibodies.
Bioworld BBS-Casein (10X), 200 mL
BSS-Casein, supplied in 10X PBS and 5% Csein. Technical Information:10X Concentrate has a working pH of 8.5 ( +/- 0.1)Formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
IBI SCIENTIFIC 3P 10X PBST (PBS W/0.5% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.5% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 10X PBST (10X PBS) with TWEEN 20 (0.5% TWEEN 20) is a concentrated sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 10X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
Zymo Research Corporation 5-mC ELISA Buffer, 250 ml
5-mC ELISA Buffer 250 ml. For use in the 5-mC DNA ELISA kit for DNA methylation quantification. Sold individually or as part of a complete ELISA kit.
Research Products International Corp PBS/Tween 20 10X Solution, 500 Milliliters
PBS/Tween 20 10X Solution is a widely used buffer solution in the life science marketplace. It is a combination of phosphate-buffered saline (PBS) and Tween 20, a nonionic detergent.
Rockland Immunochemicals BLOTTO B PRE-MIXED - for a 1X SOLUTION of Immunoanalytical Grade 1% NON-FAT DRY MILK, 1% BSA in TBS with TWEEN-20 - B553-0500
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to and during incubation with primary antibody.
LAB BIOREAGENTS StrongBlock III Buffer 250ml
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
Bioworld BSA-BLOCKING BUFFER IN TBS
BSA-Blocking Buffer in TBS (10X) Blot blocking buffer used to block non-specific binding site for antibodies on the membrane 1x Solution Contains: 3% BSA in TBS (pH 7.4); Sodium Azide (0.02%).
Bioworld Denhardt's Solution (100X), 50 mL
Denhardt's Solution, 100X Concentrate Acts as a blocking reagent in hybridizations to help prevent probe binding to open membrane space and non-target nucleic acids. Specifications:Ultra Pure GradePurity: 99.0%pH @ 25°C: 5.0-7.0 Conductivity @ 25°C (µmhos): <350 Abs @ 278nm (5% water): 0.600-0.750 DNAse: None detectedRNAse: None detectedStorage: -15 to -22°C
Bioworld Blotto in TBS, 1 L
Blotto in TBS Blot blocking buffer used to block non-specific binding for antibodies on the membrane Technical Information: 5% nonfat dry milk, 0.02% Sodium azide in TBS
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Research Products International Corp BSA Block Buf Sol wAzide 200ml
BSA Blocking Buffer Solution in TBS with Azide, 200 Milliliters