Protein Method Blocking Buffers
LAB BIOREAGENTS StrongBlock III | 500mL
Lab Bioreagents Buffers have been developed through the screening of >2000 compounds with the support of machine learning (AI) and optimized through kinetic and dynamic binding tests. StrongBlock (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlock (I, II, III) are a variety of buffers designed by different blocker components.
MilliporeSigma™ Immunoblot Blocking Reagent
For use in western blots as a blocking agent
BLOTTO and Tween 20 in PBS, with 0.02% sodium azide, Thermo Scientific™
Blocking reagent for immunohistochemistry experiments
BBS, MP Biomedicals™
Serves as a general purpose loading dye. MP Biomedicals BBS can be used with neutral DNA or RNA agarose gels.
MilliporeSigma™ Novagen™ 5% Alkali - Soluble Casein
Used as blocking reagent for Western blot applications.
Seracare Life Sciences Inc 10X DULBECCO S PBS W/ TWEEN 20
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures. pH=7.4. Conductivity controlled.
