Electrophoresis Gel Casting Reagents
Sigma Aldrich Fine Chemicals Biosciences Protein A Agarose
The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times depending on the elution condition.Protein A leakage 18 ng Protein A/ml (ELISA)Regeneration the gel can be used approximately 30 timesStructure recombinant Protein A (E. coli Mr 45 000) is covalently coupled to crosslinked 6% agarose beads 3 mg Protein A (98% pure HPLC SDS-Page)/1 ml gelProtein A immobilized.
Sigma Aldrich Fine Chemicals Biosciences Protein G Agarose
Protein G was initially isolated from G148 a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30 000Da. It interacts with all subclasses of human IgG and also binds to mouse rabbit and goat IgG.
Perkin Elmer US LLC 2500 Run Kit for Standard 75 mL, 40 Bar Digestion Vessels
2500 Run Kit for Standard 75 mL (40 Bar) Digestion Vessels for Titan MPS 16 Position Microwave Sample Preparation Systems. This kit includes: 10 Pressure Seals (N3132002) 4 Rupture Discs (N3132001) 5 Lip Seal Rings of the Gas Containment Manifold (N3134000)
Chem-Impex International, Inc. N,N'-Methylene-bis-acrylamide | 110-26-9 | MFCD00008625 | 100G
N,N'-Methylene-bis-acrylamide, 110-26-9, MFCD00008625, 100G
![Research Products International Corp SDS, 20% Solution [Sodium dodecylsulfate 20% Solution][Lauryl sulfate 20% Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-L23100-500.0.JPG-250.jpg)
Nusep Inc NUVIEW MINI GEL 10 12-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 10% 12well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Nusep Inc NUVIEW MINI GEL 12 17-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 12% 17well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Chem-Impex International, Inc. N,N'-Methylene-bis-acrylamide | 110-26-9 | MFCD00008625 | 25G
N,N'-Methylene-bis-acrylamide, 110-26-9, MFCD00008625, 25G
Crescent Chemical Co Inc AGAR AGAR SERVA KOBE I
Agar Agar SERVA Kobe I powder research grade Choice quality for bacteriology. Size - 1KG Storage Conditions - +15 °C TO +30 °C Catalog Number - 11392.03 CAS 9002-18-0
Quality Biological Inc Blocking Solution, 500ML
Blocking is a very important step of western blotting as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk to reduce the background. Nonfat dried milk is often preferred as it is inexpensive and widely available. However milk proteins are not compatible with all detection labels so care must be taken to choose the appropriate blocking solution.
Research Products International Corp Agarose, for Routine Gel Electrophoresis, Molecular Biology Grade, High Gel Strength, 250 Grams
Molecular Biology Grade Agarose. High Purity for preparative and analytical electrophoresis of nucleic acids ≥1000bp. Forms a solid gel at low concentration. Product is RNase/DNase free.
Gel Strength, 1%: ≥1200 g/cm2
Gel Strength, 1.5%: ≥2700 g/cm2
Gel Point: 35-37°C
Melting Point: 87-89°C
EEO: 0.09-0.12
Sulfate: ≥0.03%
Loss on Drying: <10%
DNA Binding: None Detected
Research Products International Corp Agarose, Low Melt Temperature, 25 Grams
Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.
Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected