Electrophoresis Gel Casting Reagents
Research Products International Corp Agarose, Low Melt Temperature, 250 Grams
Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.
Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected
Research Products International Corp bis-Acrylamide, 2% Solution, 500 Milliliters
A 2% (w/v) bis-Acrylamide solution.
![Research Products International Corp SDS, 20% Solution [Sodium dodecylsulfate 20% Solution][Lauryl sulfate 20% Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-L23100-500.0.JPG-250.jpg)
Sigma Aldrich Fine Chemicals Biosciences Protein G Agarose
Protein G was initially isolated from G148 a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30 000Da. It interacts with all subclasses of human IgG and also binds to mouse rabbit and goat IgG.
Electron Microscopy Sciences Agarose SFR™ Agarose S FR White, Fine Powder 100 GR
CAS #9012-36-6 (C₆H₁₀O₅·C₆H₈O₄)n
Melting point: ≤ 70°C
Agarose SFR is a high resolution sieving agarose with unsurpassed clarity. DNA bands differing in size by 2% can be resolved in the range of 200 – 1,000 bp. This agarose is suitable for the analysis of AFLP's (Amplified Fragment Length Polymorphisms), STR's (Short Tandem Repeats) and tetranucleotide repeats. The low melting point of agarose SFR makes it an excellent medium for analytical and preparative electrophoresis.
Alkali Scientific ASI™ Agarose HR (High-Resolution-PCR Grade), 100g
ASI™ Agrose HR is a high resolution agarose is designed for separation of fragments less than 20-800bp. Gels made with Agarose HR are suitable for high resolution of PCR products and analysis of small fragments such as STRs and AFLPs. All Benchmark agarose is manufactured under a “green” process that eliminates the use of organic solvents. They provide gels with enhanced resolution and clarity, low EEO and low background. Tested to be RNase, DNase, Protease and Endonuclease free. Supplied in 100g bottles.
Sigma Aldrich Fine Chemicals Biosciences GelGreenR Nucleic Acid Sta
GelGreen is a sensitive stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (figure 1). Thus GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen is far more sensitive than SYBR Safe (figure 2). Unlike SYBR dyes which are known to be unstable GelGreen is very stable both hydrolytically and thermally.The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization under which GelGreen is not classified as hazardous waste.Spectral PropertiesAbsorbance Standard Transilluminator (254 nm) such as a Dark ReaderEmission Lon
Quality Biological Inc TBS 10X pH 7.4, 500 mL
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc SDS 10%, 500 mL
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. ¿ SDS is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. ¿ Applications: Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE) ¿ Common component of cell lysis buffers ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc EDTA (0.5 M), pH 8.0, 500 mL
EDTA (Ethylenediaminetetraacetic acid), a metal chelator, is widely used in molecular biology as a chemical reagent to reduce nuclease activity (i.e. metalloenzyme). ¿ EDTA solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, and chelators. ¿ Applications: Typically, EDTA is added to Tris base in order to prepare Tris EDTA (TE), which can be used to reconstitute nucleic acids (i.e. DNA). ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences Acrylamide/Bis-acrylamide
Polyacrylamide gels are prepared using acrylamide and bis-acrylamide where bis-acrylamide is a cross-linking agent. On the other hand acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.