Fluorometers

Fluorometers

Instruments and respective accessories used to characterize the fluorescence a sample. Includes assays for a variety of applications.

Fluorometers or fluorospectrometers are used to identify the presence and quantity of specific molecules in a medium. The mechanisms of measurement involve a light source to excite the molecules to fluoresce and a means to isolate a specific wavelength at which to measure the light emitted.

Advantages of fluorescence include:

  • Sensitivity: Measures concentrations in the picomole and femtomole ranges (and lower)
  • Quantitative: Fluorescence signals are proportional to concentration; changes in intensity occur rapidly, making the technique suitable for in situ studies and fast processes
  • Safe: Non-destructive to biological samples, with no hazardous byproducts

Some examples of fluorescence spectroscopy areas of use include:

  • Biology
    • To measurethe fluorescence of chlorophyll (plant physiology)
    • Observe cellular processes
    • Molecule detection
  • Biochemistry
    • Cytometry and cell sorting
    • Gel electrophoresis
  • Chemistry
    • Fluorescent detectors in HPLC and other chromatography applications
    • Laser-induced fluorescence to study molecular electronic structures
    • Measure concentrations in combustion, plasma, and flow phenomena
    • Organic compound analysis
  • Environmentalmonitoring
    • Analyze dissolved organic matter in water to identify sources and paths
  • Forensics
    • Detecting fingerprints and blood
    • Analyzing fibers and other materials
  • Medicine
    • Tissue analysis for cancer detection
    • Glucose sensors
    • DNA sequencing
  • Other
    • Gemstone analysis
    • Mineral analysis
    • Crude oil residue assessments

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