PCR Equipment and Supplies

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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion High-Fidelity DNA Polymerase
GC-Rich PCR Performance	High
Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl₂ solution and DMSO Store at –20°C.
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Separate Components
Product Type	High-Fidelity PCR Kit
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
For Use With (Application)	Standard PCR,High-fidelity PCR

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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Pricing & Availability
Specifications

Physical Form	Liquid
pH	7
Chemical Name or Material	Saline-Sodium Citrate
Grade	Molecular Biology
DNase	DNase free
Recommended Storage	RT
Concentration	2.9 to 3.1 M

Thermo Scientific™ dTTP Solution (100 mM)

Thermo Scientific dTTP (2'-deoxythymidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

Thermo Scientific™ dCTP Solution (100 mM)

Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

Applied Biosystems™ VetMAX™ PRRSV 3.0 Reagents

VetMAX™ PRRSV 3.0 Reagents contain master mix, Xeno RNA control, and a primers/probes mix that enables multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) assays targeting North American (NA) and European (EU) porcine reproductive and respiratory syndrome virus (PRSSV) RNA and Xeno RNA.

Pricing & Availability

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT, TaqMan
Detection Method	TaqMan

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Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl₂

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Pricing & Availability
Specifications

Polymerase	AmpliTaq Gold DNA Polymerase
Fidelity (vs. Taq)	1X
Size (Final Product)	5 kb or less
Product Type	DNA Polymerase
Overhang	3'-A
Concentration	5 U/μL
Exonuclease Activity	5' - 3'

Thermo Scientific™ Random Hexamer Primer

Thermo Scientific Random Hexamer Primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends.

Pricing & Availability

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl₂

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Pricing & Availability
Specifications

Polymerase	AmpliTaq DNA Polymerase
Fidelity (vs. Taq)	1X
Reaction Format	Kit
Product Type	PCR Buffer
Concentration	10X
For Use With (Application)	Standard PCR

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Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum II Taq Hot-Start DNA Polymerase
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	1X
Size (Final Product)	8 kb
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Universal Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
For Use With (Application)	Hot-start PCR,Real Time PCR (qPCR)

Fisherbrand™ SYBR™ Green qPCR Master Mix

Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Content And Storage	SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Product Type	Master Mix
Form	Frozen
Reaction Speed	Fast or Standard
Label or Dye	SYBR Green,FAM
Concentration	2X
For Use With (Application)	Gene Expression,DNA Quantitation,Microbial Detection,Real Time PCR (qPCR)
PCR Method	qPCR,2-step RT-qPCR,RT-qPCR
Detection Method	SYBR

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Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid Reverse Transcriptase (200 U/μL) • 5X Reaction Buffer Store at –20°C.
Reverse Transcriptase	RevertAid
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	42°C
Technique	Reverse Transcription
Starting Material	RNA

Invitrogen™ PureLink™ PCR Purification Kit

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format	Kit
Sample Type	PCR Products
Isolation Technology	Silica Spin Column
Run Time	10 min.

Thermo Scientific™ Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Pricing & Availability
Specifications

Quantity	4 x 1.5 mL
For Use With (Application)	Standard PCR

Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion Hot Start II High-Fidelity DNA Polymerase
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start High-Fidelity PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR,High-fidelity PCR

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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ dTTP Solution (100 mM) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ dCTP Solution (100 mM) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ Random Hexamer Primer GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Invitrogen™ Platinum™ Direct PCR Universal Master Mix Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Thermo Scientific™ dTTP Solution (100 mM)

Thermo Scientific™ dCTP Solution (100 mM)

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Thermo Scientific™ Random Hexamer Primer

Invitrogen™ Platinum™ Direct PCR Universal Master Mix

Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)